Host Genotype and Edaphic Factors Cumulatively Influence the Occurrence of Siderophore-producing Bacteria Associated with Rice (Oryza sativa L.)

Phan Thi Thuy 1 , Wei-Ching Chung 2 and Li-Sen Young 3

1Faculty of Agronomy, Vietnam National University of Agriculture, Ha Noi 131000, Vietnam
2Department of Biotechnology, College of Applied Arts and Sciences, National Formosa University, Yunlin 632, Taiwan
3Tetanti AgriBiotech Inc. No. 1, Taichung city 40755, Taiwan
Received: Apr 5, 2021 /
Revised: May 20, 2022 /
Published: Mar 30, 2022

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Seed-borne rice endophytes are capable of disseminating into host plant tissues as well as to their rhizosphere. Here, we investigated the occurrence of siderophore-producing bacteria (SPB) in the seed endospheres of two distinct rice (Oryza sativa L.) cultivars, TK8 (ssp. japonica) and TCN1 (ssp. indica), and their dissemination into the rhizospheres through culture-dependent methods. Their patterns of occurrence in the rhizospheres as well as in the root and shoot tissues of 30 day-old cultivars grown in three different kinds of soils were tested. The significance of SPB on Fe sequestration of TCN1 was studied using Enterobacter sp. LS-756. TK8 seeds were found to be not only abundant in endopsheric SPB (> 10-fold), but also exhibited enhanced SPB dissemination into the rhizosphere (1.3-fold) as compared to TCN1. The proportion of endophytic SPB was consistently higher in roots than in shoots, and it was found to decline with decreasing soil pH. A similar declining trend was further evident through the analysis of SPB composition in the rhizospheric and bulk soils. LS-756-inoculated TCN1 seedlings under low availability of Fe showed 32%, 178%, and 368% increases in Fe, chlorophyll, and chlorophyll b contents as compared to the uninoculated controls. Thus, the occurrence of seed-borne endophytic SPB and their dissemination into the rhizosphere vary significantly according to the rice genotype. Higher co-occurrence of SPB in the rhizosphere and internal root tissues of rice plants grown under Fe-limited conditions and the enhanced Fe uptake due to SPB inoculation substantiated their potential involvement in Fe sequestration.

Keywords: Seed endosphere, rhizosphere, dissemination, siderophore, Fe sequestration

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How to Cite
Thuy, P., Chung, W.-C., & Young, L.-S. (2022). Host Genotype and Edaphic Factors Cumulatively Influence the Occurrence of Siderophore-producing Bacteria Associated with Rice (Oryza sativa L.). Vietnam Journal of Agricultural Sciences, 5(1), 1313-1325.


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