Date Received: Aug 21, 2018
Date Accepted: Aug 21, 2018
Date Published: Aug 21, 2018
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ENGINEERING AND TECHNOLOGY
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Canh, N., Thom, D., & Huyen, N. (2018). Characterization and Identification of a Streptomyces Strain with Biocontrol Activity against Aeromonas Hydrophila Causing Haemorrhage Disease in Fish. Vietnam Journal of Agricultural Sciences, 1(1), 52–59. https://doi.org/10.31817/vjas.2018.1.1.06
Characterization and Identification of a Streptomyces Strain with Biocontrol Activity against Aeromonas Hydrophila Causing Haemorrhage Disease in Fish
,
Dinh Thi Thom
1
,
Nguyen Thanh Huyen
1
Keywords
Fish, Sequence analysis, 16S rRNA, Aeromonas hydrophila, Streptomyces sp
Abstract
In this study, experiments were performed to identify and screen Streptomyces strains that are antagonistic to Aeromonas hydrophila, which causes hemorrhagic disease in fishes. Among 80 isolates, 3 strains capable of antagonizing Aeromonas hydrophila by the agar diffusion plate method were obtained. The strain numbered 1083 had a strong activity with a 25 mm diameter clear zone of bacteria. This strain showed grey colonies after 7 days of incubation. After 7 days of incubation, the grey colonies had white borders, produced soluble pigments on the medium, grew well at 30°C and neutral pH, and adapted well to medium containing a high salt concentration. Strain 1083 was able to utilize different sources of carbon and nitrogen. Sequence analysis of 16S rRNA showed that strain 1083 had a similarity of 99% compared to Streptomyces antibioticus. Based on morphological, physiological, and biochemical characteristics, as well as molecular biological analysis, strain 1083 was identified as Streptomyces antibioticus.
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2. Altschul S. F., Gish W., Miller W., Myers E. W. and Lipman D. J. (1990). Basic local alignment search tool. Journal of Molecular Biology. Vol 215. pp. 403-410.
3. Dharmaraj S., Ashokkumar B. and Dhevendaran K. (2009). Isolation of marine Streptomyces and the evaluation of its bioactive potential. African Journal of Microbiology Research. Vol 4 (4). pp. 240-248.
4. FAO (2014). The state of world fisheries and aquaculture 2014: Opportunities and challenges. Retrieved on January 1, 2017 at http://www.fao.org/3/a-i3720e.pdf.
5. Julia P. G. and Christina A. K. (2008). Cross-Kingdom Amplification Using Bacteria-Specific Primers: Complications for Studies of Coral Microbial Ecology. Applied and Environmental Microbiology. Vol 74 (24). pp. 7828-7831.
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7. Marmur J. (1961). A Procedure for the Isolation of Deoxyribo-nucleic Acid from Micro-Organism. Journal of Molecular Biology. Vol 3 (2). pp. 208-218.
8. Miyadoh S., Tsuchizaki N., Ishikawa J. and Hotta K. (2016). Digital Atlas of Streptomyces. The Society for Streptomyces Japan, Asakura Co.
9. Mohana S. and Radhakrishnan M. (2014). Streptomyces sp MA7 isolated from mangrove rhizosphere sediment effective against Gram negative bacterial pathogens. International Journal of PharmTech Research (0974 - 4290). Vol 6 (4). pp. 1259-1264.
10. Rasmussen-Ivey C. R., Hossain M. J., Odom S. E., Terhune J. S., Hemstreet W. G., Shoemaker C. A., Zhang D., Xu D-H., Griffin M. J., Liu Y-J., Figueras M. J., Santos S. R., Newton J. and Cand M. R. (2016). Classification of a hypervirulent aeromonas hydrophila pathotype responsible for epidemic outbreaks in warm-water fishes. Frontiers in Microbiology. Vol 7. pp. 1615. doi: 10.3389/fmicb.2016.01615.
11. Samira T. L., Gisele V. G. and Mateus M. C. (2012). Molecular characterization of virulence factors in Aeromonas hydrophila obtained from fish. Pesquisa Veterinária Brasileira. Vol 32 (8). pp. 701-706.
12. Selvakumar D. (2010). Antagonistic potential of marine Actinobacteria against fish and shellfish pathogens. Turkish Journal of Biollogy. Vol 35 (2011). pp. 303-311.
13. Selvakumar D., Arun K., Suguna S., Kumar D. and Dhevendaran K. (2010). Bioactive potential of Streptomyces against fish and shellfish pathogens. Iranian Journal of Microbiology. Vol 2 (3). pp. 157-164.
14. Shirling E. B. and Gottlieb D. (1966). Methods for characterization of Streptomyces species. International Journal of Systematic Bacteriology. Vol 16. pp. 313-340.
15. Trang T. T., Dung N. T. and Hoai T. D. (2017). Isolation of bacteria caused Hemorrhagic disease for catfish (Ictalurus punctatus) in the Northern parts of Vietnam. Vietnam Journal of Agriculture Science. Vol 15 (4). pp. 446-454.
16. Vivekanandhan G., Hatha A. A. M. and Lakshmanaperumalsamy P. (2004). Prevalence of Aeromonas hydrophila in fish and prawns from the seafood market of Coimbatore, South India. Food Microbiology. Vol 22 (1). pp. 133-137.