Generation of Synthetic Peptide-Specific Antibody for the Development of A Southern Rice Black-Streaked Dwarf Virus Diagnostic Test

Date Received: May 18, 2020

Date Published: Oct 29, 2021

DOI: https://doi.org/10.31817/vjas.2021.4.3.08

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Issue: Vol. 4 No. 3 (2021): Vietnam Journal of Agricultural Sciences

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ENGINEERING AND TECHNOLOGY

How to Cite:

Hanh, D., Minh, N., Cuu, N., Huong, P., Hoi, P., & Phuong, N. (2021). Generation of Synthetic Peptide-Specific Antibody for the Development of A Southern Rice Black-Streaked Dwarf Virus Diagnostic Test. Vietnam Journal of Agricultural Sciences, 4(3), 1176–1184. https://doi.org/10.31817/vjas.2021.4.3.08

Generation of Synthetic Peptide-Specific Antibody for the Development of A Southern Rice Black-Streaked Dwarf Virus Diagnostic Test

Do Thi Hanh (*) 1 , Nguyen Anh Minh 2 , Nguyen Van Cuu 2 , Phung Thi Thanh Huong 2 , Pham Xuan Hoi 2   , Nguyen Duy Phuong 2

  • Corresponding author: phuongnd.bio@gmail.com
  • 1 Faculty of Chemical Technology, Hanoi University of Industry, Hanoi 129000, Vietnam
  • 2 Department of Molecular Pathology, Agricultural Genetics Institute, Hanoi 123100, Vietnam

    • Keywords

    Polyclonal antibody, rice, P10 envelope protein, SRBSDV

    Abstract


    Southern rice black-streaked dwarf virus (SRBSDV) causes severe epidemical disease on rice with the infected area up to millions of hectares in South China and North and Central of Vietnam. So far, there are no effective, cheap, quick, and practicable methods for diagnosing SRBSDV. The conventional RT-PCR technique is the most popular method for detecting SRBSDV with high accuracy. However, it is hard to apply this method for large-scale SDBSDV diagnosis because of the requirements of expensive reagents and instruments, as well as complex procedures. Meanwhile, SRBSDV diagnostic techniques based on antigen detection have outstanding advantages due to their low cost, easy manipulation, and wide application possibility. Today, there are still no commercially available specific antibodies to SRBSDV. In a previous study, to develop the SRBSDV diagnostic technique by the ELISA technique, a SRBSDV specific antibody was generated by a recombinant P10 envelope protein (66kDa), which has a titer of 1:5,000. In this study, we continued to study the production of SRBSDV specific polyclonal antibodies from small antigen–rich peptides from the SRBSDV P10 envelope protein. The resulting purified antibody can specifically bind to the P10 protein and at the diluted concentration of 1:100,000 it can detect SRBSDV in infected rice samples via the dot-blot technique. Our research results open up new opportunities for proactive antibodies to develop a SRBSDV membrane rapid diagnostic kit.

    References

    Amero S. A., James T. C. & Elgin C. R. (1994). Production of antibodies using protein in gel bands. Basic Protein and Peptide Protocols (Humana Press, editor). Springer. 32: 717-720.

    Anh T. H., Zhang H. M., Yang J., Chen J. P., Hebrard E., Zhou G. H., Vien N. V. & Cheng J. A. (2011) Identification, characterization, and distribution of southern rice black-streaked dwarf virus in Vietnam. Plant Disease. 95: 1063-1069.

    Cuong H. V., Hai N. V., Man V. T. & Masaru M. (2010). Rice dwarf disease in North Vietnam in 2009 is caused by southern rice black-streaked dwarf virus (SRBSDV). Bulletin of the Institute of Tropical Agriculture, Kyushu University: 85-92.

    Guo H. Z., Jung W. J., Jiang C. D., Peng L., Lin X. D. & Guang Z. S. (2008). Southern rice black–streaked dwarf virus: A new proposed Fijivirus species in the family Reoviridae. Chinese Science Bulletin. 53: 3677-3685.

    Do Thi Hanh, Pham Thi Van & Pham Xuan Hoi (2015). Generation of polyclonal antibody against SRBSDV by recombinant protein. Vietnam Journal Agriculture and Rural Development. 278(1): 38-43 (in Vietnamese).

    Matshukura K., Towata T., Sakai J., Onuki M. & Matsumura M. (2013). Dynamics of southern rice black-streaked dwarf virus in rice and implication for virus acquisition. Journal of Phytopathology. 103: 509-512.

    Nguyen Hoang Quang, Do Thi Hanh, Pham Thi Van, Tran Thi Nhu Hoa, Ha Viet Cuong & Pham Xuan Hoi (2013). Sequence analysis of S10 segment of virus isolates causing black-streaked dwarf disease on rice in Vietnam. Journal of Vietnam Agricultural Science and Technology. 2: 26-31 (in Vietnamese).

    Sambrook J. & Russell D. W. (2001). Molecular Cloning: A Laboratory Manual (3rd ed.). Cold Spring Harbor Laboratory. Cold Spring Harbor, NY.

    Pham Thanh Tam, Pham Thi Van, Nguyen Hoang Quang, Nguyen Duy Phuong & Pham Xuan Hoi (2013). Expression and purification of outer capsid protein P10 of Southern Rice Black-Striked Dwarf virus. Vietnam Journal Agriculture and Rural Development. 231(2): 35-40 (in Vietnamese).

    Ngo Vinh Vien, Pham Thi Vuong, Nguyen Nhu Cuong, Ta Hoang Anh, Nguyen Thi Me, Phan Bich Thu, Pham Hong Hien & Ha Viet Cuong (2009). Diagnostic results for rice black-streak dwarf virus in some northern provinces of Vietnam. Journal of Plant Protection. 6: 8-18 (in Vietnamese).

    Wang Q., Yang J., Zhou G. H., Zhang H. M., Chen J. P. & Adams M. J. (2010). The complete genome sequence of two isolates of southern rice black-streaked dwarf virus, a new member of the genus Fijivirus. Journal of Phytopathology. 158: 733-737.

    Wang Z., Yu D., Li X., Zeng M., Chen Z., Bi L., Liu J., Jin L., Hu D., Yang S. & Song B. (2012). The development and amplification of a Dot-ELISA assay for diagnosis of southern rice black-streaked dwarf virus in the field. Viruses. 4: 167-183.

    Yin X., Xu F., Zheng F., Li X., Liu B. & Zhang C. (2011). Molecular characterization of segments S7 to S10 of a southern rice black-streaked dwarf virus isolate from maize in Northern china. Virologica Sinica. 26(1): 47-53.

    Zhang H. M., Yang J., Chen J. P. & Adams M. J. (2008). A black-streaked dwarf disease on rice in China is caused by a novel fijivirus. Archives of Virology. 153: 1893-1898.

    Zhang S., Zhang D., Liu Y., Luo X., Cheng J. & Peng J. (2013). Development of a real-time RT-PCR method for detection and qualification of southern rice black-streaked dwarf virus in rice. Journal of Plant Pathology and Microbiology. 4(7): 1-4.