Characterization of Variovorax Strain C6d Isolated from the Algae-bacteria Consortia

Date Received: Sep 24, 2020

Date Published: Oct 29, 2021

DOI: https://doi.org/10.31817/vjas.2021.4.3.07

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Issue: Vol. 4 No. 3 (2021): Vietnam Journal of Agricultural Sciences

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NATURAL RESOURCES AND ENVIRONMENT

How to Cite:

Hoan, V. (2021). Characterization of Variovorax Strain C6d Isolated from the Algae-bacteria Consortia. Vietnam Journal of Agricultural Sciences, 4(3), 11168–1175. https://doi.org/10.31817/vjas.2021.4.3.07

Characterization of Variovorax Strain C6d Isolated from the Algae-bacteria Consortia

Vu Thi Hoan (*) 1

  • Corresponding author: thihoan.vu@gmail.com
  • 1 Faculty of Natural Resources and Environment, Vietnam National University of Agriculture, Hanoi 131000, Vietnam

    • Keywords

    Variovorax, Characterization, algae-bacteria consortia

    Abstract


    The rhizosphere microorganisms can form beneficial, pathogenic, or neutral relationships. These relationships can promote plant growth and productivity. Among them, a number of Variovorax isolates from the rhizosphere were isolated. Bacteria Variovorax strain C6d (AB552893) was isolated from the non axenic culture of Chlorella spp., C6. The cell was Gram-negative, motile, non-spore-forming, short and rod-shaped (0.5-1.0x1.5-2.0µm). Colonies were in white colour after 7 days on 10-fold diluted Nutrient Broth. The strain was able to tolerate NaCL to 1.0% but not to 4.0% of NaCl. It grew quite well at temperatures ranging from 10°C to 37°C, yet did not show any growth at 4°C and 42°C. The dominant isoprenoid quinone was ubiquinone 8 (Q8). The major fatty acid composition of this strain was summed feature 3, 16:0 and 18:1:w7. The DNA G+C content of strain C6d was 70.4 mol%.

    References

    Ashida N., Ishii S., Hayano S., Tago K., Tsuji T., Yoshimura Y., Otsuka S. & Senoo K. (2010). Isolation of functional single cells from environments using a micromanipulator: application to study denitrifying bacteria. Applied Microbial. Biotechnology. 85: 1211-1217.

    Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seid-man J. G., Smith J. A. & Struhl K. (1995). Preparation and analysis of DNA. In: Ausubel F.M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A. & Struhl K. (Eds.) Current Protocols in Molecular Biology. Wiley, New York: 2-11 and 109-111.

    Cole J. R., Chai B., Farris R. J., Wang Q., Kulam-Syed-Mohideen A. S., McGarrell D. M., Bandela A. M., Cardenas E., Garrity G. M. & Tiedje J. M. (2007). The ribosomal database project (RDP-II): introducing my RDP space and quality controlled public data. Nucleic Acids. 35: 169-172.

    Collins M. D. & Jones D. (1981). Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implication. American Society for Microbiology. 45(2): 316-354.

    Futamata H., Nagano Y., Watanabe K. & Hiraishi A. (2005). Unique kinetic properties of phenol-degrading Variovorax strains responsible for efficient trichloroethylene degradation in a chemostat enrichment culture. Applied Environmental Microbiology. 71: 904-911.

    Gerhardt P., Murray R. G. E., Wood W. A. & Krieg N. R. (1994). Methods for General and Molecular Bacteriology. Washington, DC: American Society for Microbiology.

    Han J. I, Choi H. K, Lee S. W, Paul M. O, Kim J., Sarah L. R, Kim T. G., Jennifer O. N, Jared R. L , Sang Y. L, Hur C. G, Jim C. S, Galina O., Goodwin L. & Han C. (2011). Complete genome sequence of the metabolically versatile plant growth-promoting endophyte Variovorax paradoxus S110. Journal of Bacteriology. 193: 1183-1190.

    Ichimura T. (1971). Sexual cell division and conjugation-papilla formation in sexual reproduction of Closterium strigosum. In: by Nishizawa K. (Ed.). Proceedings of the 7th International Seaweed Symposium, University of Tokyo Press, Tokyo: 208-214.

    Im W. T., Liu O. M, Lee K. J, Kim S. Y., Lee S. T. & Yi T. H. (2010). Variovorax ginsengisoli sp. nov., a denitrifying bacterium isolated from soil of a ginseng field. International Journal of Systematic and Evolutionary Microbiology. 60: 1565-1569.

    Jennifer A. C. (2019). Investigating the Functions of the Plant-associated Genus Variovorax in the Populus Rhizosphere. Masters Theses. University of Tennessee, Knoxville.

    Kim B. Y., Weon H. Y., Yoo S. H., Lee S. Y., Kwon S. W., Go S. J. & Stackebrandt E. (2006). Variovorax soli sp. nov., isolated from greenhouse soil. International Journal of Systematic and Evolutionary Microbiology. 56: 2899-2901.

    Kristina M. M., Zheng H., Tekle T. F., Ronald J. P., David E. G. & Jim C. S. (2017). Iron-Dependent Enzyme Catalyzes the Initial Step in Biodegradation of N-Nitroglycine by Variovorax sp. Strain JS1663. Applied Environmental Microbiology. 83(17). DOI: 10.1128/AEM.00457-17.

    Larkin M. A., Blackshields G., Brown N. P., Chenna R., Mcgettigan P. A., Mcwilliam H., Valentin F., Wallace I. M., Wilm A., Lopez R., Thompson J. D., Gibson T. J. & Higgins D. G. (2007). Clustal W. and Clustal X. version 2.0. Bioinformatics. 23: 2947-2948.

    Mesbah M., Premachandran U. & Whitman W. B. (1989). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. International Journal of Systematic Bacteriology. 39: 159-167.

    Miwa H., Ahmed I., Yoon J., Yokota A. & Fujiwara T. (2008). Variovorax boronicumulans sp. nov., a boron-accumulating bacterium isolated from soil. International Journal of Systematic and Evolutionary Microbiology. 58: 286-289.

    Otsuka S., Abe Y., Fukui R., Nishiyama M. & Senoo K. (2008a). Presence of previously undescribed bacterial taxa in non-axenic Chlorella cultures. The Journal of General and Applied Microbiology. 54: 187-193.

    Saito T., Ishii S., Otsuka S., Nishiyama M. & Senoo K. (2008). Identification of novel Betaproteobacteria in succinate-assimilating population in denitrifying rice paddy soil by using stable isotope probing. Microbes and Environments. 23: 192-200.

    Satola B., Wubbeler J. H. & Steinbuchel A. (2013). Metabolic characteristics of the species Variovorax paradoxus. Applied Microbiology and Biotechnology. 97: 541-560

    Tuan N. M., Ngoc T. H. & Kim J. (2018). Proposal of three novel species of soil bacteria, Variovorax ureilyticus, Variovorax rhizosphaerae, and Variovorax robiniae, in the family Comamonadaceae. Journal of Microbiology. 56: 485-492.

    Yoon J. H., Kang S. J. & Oh T. K. (2006). Variovorax dokdonensis sp. nov., isolated from soil. International Journal of Systematic and Evolutionary Microbiology. 56: 811-814.

    Vu H. T., Otsuka S., Ueda H. & Senoo K. (2010). Cocultivated bacteria can increased or decreased the culture lifetime of Chlorella vulgaris. The Journal of General and Applied Microbiology. 56(5): 413-418.

    Willems A., De Ley J., Gillis M. & Kersters K. (1991). Comamonadaceae, a new family encompassing the Acidovorans rRNA complex, including Variovorax paradoxus gen. nov., comb. nov., for Alcaligenes paradoxus (Davis 1969). International Journal of Systematic Bacteriology. 41: 445-450.