Study on Stem Cutting Propagation of Gynostemma Pentaphyllum (Thunb.) Makino in Hoa Binh Province

Date Received: Oct 28, 2020

Date Published: Aug 10, 2021

DOI: https://doi.org/10.31817/vjas.2021.4.2.04

Views

2090

Download

364

How to Cite:

Phip, N., Mai, N., Hai, N., & Thom, N. (2021). Study on Stem Cutting Propagation of Gynostemma Pentaphyllum (Thunb.) Makino in Hoa Binh Province. Vietnam Journal of Agricultural Sciences, 4(2), 1034–1042. https://doi.org/10.31817/vjas.2021.4.2.04

Study on Stem Cutting Propagation of Gynostemma Pentaphyllum (Thunb.) Makino in Hoa Binh Province

Ninh Thi Phip (*) 1 , Nguyen Phuong Mai 1 , Nguyen Thi Thanh Hai 1   , Nguyen Mai Thom 1

  • Corresponding author: ntphip@vnua.edu.vn
  • 1 Faculty of Agronomy, Vietnam National University of Agriculture, Hanoi 131000, Vietnam

    • Keywords

    Gynostemma pentaphyllum, stem cutting, propagation

    Abstract


    Gynostemma pentaphyllum (Thunb.) Makino is a medicinal plant distributed mostly in the mountainous areas of Vietnam. G. pentaphyllum contains more than 189 types of saponin which can be used for the treatment of various diseases. Currently, due to over-exploitation, this plant species is under threaten. Our research aimed to study a suitable method for plant propagation by stem cutting techniques to promote the growth and development of G. pentaphyllum. Six experiments were conducted to (i) evaluate the established seedling rates of different G. pentaphyllum varieties, and choose the best variety to (ii) evaluate the established seedling rate influenced by different factors, viz. stem cutting position, cutting season, growing in the selected substrates, stem cutting length, number of buds per stem, and application of α-NAA. The results showed that among five varieties, G2 which was collected in Mai Chau district disclosed the highest ability to shooting, the highest number of roots per stem cutting, the shortest nursery days, the highest shooting rate, and the highestestablished seedling rate. We also determined that using middle-aged plants for stem cuttings, at 15-20cm in length, with 2 nodes and grown in Spring and Winter cropping seasons, using growing substrates with a mixture of 50% of black sand and 50% of soil, and plus α-NAA 50ppm in 3-5min produced the highest explant rate. The established seedlingsat the time of explant were 15-20cm in height, containing 4-5 leaves and at least 9-10 roots per cutting, and were 30-32 days.

    References

    Amri E., Lyaruu H. V. M. & Nyomora A. S. (2010). Vegetative propagation of African Blackwood (Dalbergia melanoxylon Guill. & Perr.): effects of age of donor plant, IBA treatment and cutting position on rooting ability of stem cuttings. New Forests. 39: 183-194.

    Blumert M. & Liu J. L. (1999). Jiaogulan China’s ‘‘immortality’’ herb. Badger, USA: Torchlight Publishing Inc.

    Chen J. (2000). A novel Cdc2-related protein kinase, is required for hyphal development and virulence in Candida albicans. Molecular Cell Biology. 20(23): 696-708.

    Do Tat Loi (2004). Medicinal plants and medicine in Vietnam. Medicine Publisher, Ha Noi (in Vietnamese).

    Guo W. Y. & Wang W. X. (1993). Cultivation and utilization of Gynostemma pentaphyllum. Publishing House of Electronics, Science and Technology University, Beijing: 1-26.

    Hartman H. T. & Kester D. E. (1983). Plant propagation: Principles and Practices (4th ed.), Prince-Hall International, London, 727.

    Li Y., Lin W., Huang J., Xie Y. & Ma W. (2016). Anti-cancer effects of Gynostemma pentaphyllum (Thunb.) Makino (Jiaogulan). Chinese Medicine. 11:43. DOI: 10.1186/s13020-016-0114-9.

    Ninh Thi Phip (2013). Techniques for improving vegetative propagation rate of (Polyscias fruticosa (L.) harms. Journal of Scientific Research and Development. 11(2): 168-173.

    Satpal M., Rawat S. S. & Singh K. K. (2014). Effect of various concentrations of IBA, type of cuttings and planting time on the rooting of cuttings of lemon (Citrus limon Burm.) cv. PantLemon-1 under valley conditions of Garhwalhimalaya. International Journal Current Research. 6(12): 10974-10976.

    Singh K. K., Rawat J. M. S. & Tomar Y. K. (2011). Influence of Iba on Rooting Potential of Torch Glory Bougainvillea glabra During Winter Season. Journal of Horticultural Science and Ornamental Plants. 3(2): 162-165.

    Tanner M. A., Bu X., Steimle J. A. & Myers P. R. (1999). The directrelease of nitric oxide by gypenosides derived from the herb Gynostemma pentaphyllum. Nitric Oxide. 3(5): 359-365.

    Totaan D. (2019). Effects of Stem Cutting Section and Indole–3–Butyric Acid on the Vegetative Propagation of Antidesma Bunius (Linn) Spreng. Retrieved from https://ssrn.com/abstract=3464989 on December 12, 2019.

    Wu Y., Zhang Y., Wu J. A., Lowell T., Gu M. & Yuan C. S. (1998). Effects of Erkang, a modified formulation of Chinese folk medicine Shi-Quan-Da-Bu-Tang, on mice. Journal Ethnopharmacol. 61: 153-159.