Additive and Dominance Effects of MC4R and PIT1 Polymorphisms on Production and Carcass Traits in Duroc Pigs

Date Received: Apr 13, 2022

Date Published: Dec 30, 2022

DOI: https://doi.org/10.31817/vjas.2022.5.4.03

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Issue: Vol. 5 No. 4 (2022): Vietnam Journal of Agricultural Sciences

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ANIMAL SCIENCE – VETERINARY MEDICINE – AQUACULTURE

How to Cite:

Bo, H., Hung, N., Manh, N., Vinh, N., & Luc, D. (2022). Additive and Dominance Effects of MC4R and PIT1 Polymorphisms on Production and Carcass Traits in Duroc Pigs. Vietnam Journal of Agricultural Sciences, 5(4), 1638–1644. https://doi.org/10.31817/vjas.2022.5.4.03

Additive and Dominance Effects of MC4R and PIT1 Polymorphisms on Production and Carcass Traits in Duroc Pigs

Ha Xuan Bo (*) 1 , Nguyen Van Hung 2 , Nguyen Xuan Manh 2 , Nguyen Thi Vinh 1   , Do Duc Luc 1

  • Corresponding author: hxbo@vnua.edu.vn
  • 1 Faculty of Animal Science, Vietnam National University of Agriculture, Hanoi 131000, Vietnam
  • 2 Dabaco Nucleus Breeding Pig Company, Bac Ninh 220000, Vietnam

    • Keywords

    MC4R, PIT1, polymorphisms, additive effect, Duroc pigs

    Abstract


    This study aimed to estimate the allelic and genotypic frequencies of single nucleotide polymorphisms (SNPs) of MC4R (melanocortin-4-receptor gene) and PIT1 (pituitary specific transcription factor-1) genes, and their additive dominance genetic effects on production and carcass traits in Duroc pigs. A total of 2170 tail tissue samples (1728 gilts and 442 male pigs) were collected from Dabaco Nucleus Breeding Pig Company, Bac Ninh province, Vietnam from January 2017 to June 2020. Polymorphisms in the MC4R and PIT1 genes were detected using the PCR-RFLP method. For MC4R, the frequencies of alleles A and G were 0.430 and 0.570, respectively. For PIT1, the frequencies of alleles A and B were 0.460 and 0.540, respectively. The genotype frequency distribution of the MC4R gene was in Hardy-Weinberg equilibrium (P = 0.375), but the genotype frequency distribution of PIT1 (P <0.001) was not. The MC4R and PIT1 polymorphisms were significantly associated with final body weight and average daily gain (P <0.001). Significant additive effects were also found (P <0.05) for the two traits. The selection of individuals with the MC4R genotype AA and PIT1 genotype AA could improve final body weight and average daily gain in Duroc pigs.

    References

    Bo H. X., Trang L. T., Hung N. V., Manh T. X. & Luc D. D. (2019a). Association between polymorphisms of MC4R and PIT1 genes with sperm quality traits of Duroc. Proceedings of National Conference on Animal and Veterinary Science, Ho Chi Minh city, Vietnam: 74-79.

    Bo H. X., Trang L. T., Hung N. V., Manh T. X., Canh N. X. & Luc D. D. (2019b). Polymorphisms of MC4R and PIT1 genes in Duroc pig populations. Journal of Animal Husbandry and Technics. 243: 2-6.

    Brunsch C., Sternstein I., Reinecke P. & Bieniek J. (2002). Analysis of associations of PIT1 genotypes with growth, meat quality, and carcass composition traits in pigs. Journal of Applied Genetics. 43(1): 85-92.

    Bruun C., Jørgensen C. B., Nielsen V. H., Andersson L. & Fredholm M. (2006). Evaluation of the porcine melanocortin 4 receptor (MC4R) gene as a positional candidate for a fatness QTL in a cross between Landrace and Hampshire. Animal Genetics. 37(4): 359-362.

    Chao Z., Wang F., Deng C.-Y., Wei L.-M., Sun R.-P., Liu H.-L., Liu Q.-W. & Zheng X.-L. (2012). Distribution and linkage disequilibrium analysis of polymorphisms of MC4R, LEP, H-FABP genes in the different populations of pigs, associated with economic traits in DIV 2 line. Molecular Biology Reports. 39(5): 6329-6335.

    Dung C. A., Thao L. T. T., Anh B. P. N., Anh H. Q., Thanh N. D. & Minh H. N. (2014). Analysis of PIT1 polymorphism in pigs and effect on growth performance, meat quality. Retrieved from http://iasvn.org/upload/files/P2N0Y7Z4QA20-7-Gen%20PIT-1%20tren%20Heo-ok.pdf on November 30, 2018.

    Culbertson M., Mabry J., Misztal I., Gengler N., Bertrand J. & Varona L. (1998). Estimation of dominance variance in purebred Yorkshire swine. Journal of Animal Science. 76(2): 448-451.

    Davoli R., Braglia S., Valastro V., Annarratone C., Comella M., Zambonelli P., Nisi I., Gallo M., Buttazzoni L. & Russo V. (2012). Analysis of MC4R polymorphism in Italian Large White and Italian Duroc pigs: association with carcass traits. Meat Science. 90(4): 887-892.

    Dvořáková V., Stupka R., Šprysl M., Čítek J., Okrouhlá M., Kluzáková E. & Kratochvílová H. (2011). Effect of the missense mutation Asp298Asn in MC4R on growth and fatness traits in commercial pig crosses in the Czech Republic. Czech Journal of Animal Science. 56: 176-180.

    Franco M. M., Antunes R. C., Oliveira K. M. d., Pereira C. D., Biase F. H., Nunes F. d. M. F. & Goulart L. R. (2005a). Association of PIT1 gene polymorphism with growth hormone mRNA levels in pig pituitary glands. Genetics and Molecular Biology. 28(1): 16-21.

    Franco M. M., Antunes R. C., Silva H. D. & Goulart L. R. (2005b). Association of PIT1, GH, and GHRH polymorphisms with performance and carcass traits in Landrace pigs. Journal of Applied Genetics. 46(2): 195-200.

    Galve A., Burgos C., Silió L., Varona L., Rodríguez C., Ovilo C. & López-Buesa P. (2012). The effects of leptin receptor (LEPR) and melanocortin-4 receptor (MC4R) polymorphisms on fat content, fat distribution and fat composition in a Duroc× Landrace/Large White cross. Livestock Science. 145(1-3): 145-152.

    Hong J., Kim D., Cho K., Sa S., Choi S., Kim Y., Park J., Schmidt G. S., Davis M. E. & Chung H. (2015). Effects of genetic variants for the swine FABP3, HMGA1, MC4R, IGF2, and FABP4 genes on fatty acid composition. Meat Science. 110: 46-51.

    Houston R., Cameron N. & Rance K. (2004). A melanocortin‐4 receptor (MC4R) polymorphism is associated with performance traits in divergently selected Large White pig populations. Animal Genetics. 35(5): 386-390.

    Kim G.-W., Yoo J.-Y. & Kim H.-Y. (2014). Association of the genotype of POU1F1 intron 1 with carcass characteristics in crossbred pigs. Journal of Animal Science and Technology. 56(1): 25.

    Kim K. S., Larsen N., Short T., Plastow G. & Rothschild M. F. (2000). A missense variant of the porcine melanocortin-4 receptor (MC4R) gene is associated with fatness, growth, and feed intake traits. Mammalian Genome. 11(2): 131-135.

    Ministère des classes moyennes et de l’agriculture de Belgique (1999). Arrêté ministériel relatif au classement des carcasses de porcs, 03 mai 1999. Retrieved from http://www.ejustice.just.fgov.be/doc/rech_f.htm on May 12, 2020 (in French).

    Muñoz G., Alcázar E., Fernández A., Barragán C., Carrasco A., De Pedro E., Silió L., Sánchez J. & Rodríguez M. C. (2011). Effects of porcine MC4R and LEPR polymorphisms, gender and Duroc sire line on economic traits in Duroc× Iberian crossbred pigs. Meat Science. 88(1): 169-173.

    Noi N. V., Phung T. V. & Hoan T. X. (2010). Analysis of the MC4R andGHRH genes polymorphism of Thai wild boar and crossbred Thai wild boar and Pac Nam local sows. Journal of Animal Husbandry and Technics. 25: 71-76.

    Piórkowska K., Ropka-Molik K., Oczkowicz M., Różycki M. & Żukowski K. (2013). Association study of PIT1 and GHRH SNPs with economically important traits in pigs of three breeds reared in Poland. Animal Science Papers and Reports. 31(4): 303-314.

    Piórkowska K., Tyra M., Rogoz M., Ropka-Molik K., Oczkowicz M. & Różycki M. (2010). Association of the melanocortin-4 receptor (MC4R) with feed intake, growth, fatness and carcass composition in pigs raised in Poland. Meat Science. 85(2): 297-301.

    Stančeková K., Vašíček D., Peškovičová D., Bulla J. & Kubek A. (1999). Effect of genetic variability of the porcine pituitary‐specific transcription factor (PIT‐1) on carcas traits in pigs. Animal Genetics. 30(4): 313-315.

    Tempfli K., Soimon Z., Kovács B., Posgay M. & Bali P. Á. (2015). PRLR, MC4R and LEP polymorphisms, and ADIPOQ, A-FABP and LEP expression in crossbred Mangalica pigs. The Journal of Animal and Plant Sciences. 25(6): 1746-1752.

    Thuy H. T., Nhan G. T. T. N., Mai P. T. P., Thuy T. T. T., Nam L. Q., Thuy D. P., Hung N. V., Manh T. X., Soan D. V. & Lan P. D. (2019). Associations of some candidate gene polymorphisms with growth traits in Duroc pigs. Livestock Research for Rural Development. 31(10): Article #158.

    Youssao A. K. I., Verleyen V. & Leroy P. L. (2002). Prediction of carcass lean content by real-time ultrasound in Pietrain and negative-stress Pietrain. Journal Animal Science. 75: 25-32.

    Yu T.-P., Tuggle C. K., Schmitz C. & Rothschild M. F. (1995). Association of PIT1 polymorphisms with growth and carcass traits in pigs. Journal of Animal Science. 73(5): 1282-1288.