Expression of Zinc Finger Protein Zat12 from Arabidopsis thaliana in Escherichia coli

Le Thi Tuyet Cham 1 , Vu Ngoc Thang 1 , Tran Anh Tuan 1 and Vu Thi Thuy Hang 1

1Faculty of Agronomy, Vietnam National University of Agriculture, Hanoi 131000, Vietnam
Received: Oct 26, 2018 /
Revised: Aug 15, 2020 /
Published: Jul 22, 2020

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Abstract

The C2H2 zinc finger protein ZAT12 has been classified as a plant core abiotic stress response gene in the early  response to multiple stresses. ZAT12 links the iron deficiency and oxidative stress responses through the direct interaction with/and negative regulation of a central regulator - FIT. For further research on the regulation of the ZAT12 protein in planta, a huge quantity of ZAT12 proteins is required to inject into mice for the generation of ZAT12 antiserum. In this study, the gene encoding the ZAT12 protein from Arabidopsis thaliana was cloned into the expression vector - pETBlue-2 and then overexpressed in E. coli T7. A high expression level was indicated by SDS-PAGE. Immunoblot demonstrated successful expression using a bacterial expression system.

Keywords: ZAT12, zinc finger protein, ZAT12 expression

Article Details

How to Cite
Cham, L., Thang, V., Tuan, T., & Thuy Hang, V. (2020). Expression of Zinc Finger Protein Zat12 from Arabidopsis thaliana in Escherichia coli. Vietnam Journal of Agricultural Sciences, 3(1), 504-511. https://doi.org/10.31817/vjas.2020.3.1.03

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