Polymorphism Candidate Genes of Lien Minh Chickens

Binh Nguyen Thi Tran 1 , Duc Huu Nguyen 1 , Quy Duc Vu 1 , Giang Thu Pham 2 , Linh Manh Nguyen 3 , Ngoc Thuy Thi Dinh 4 and Dieu Thuy Thi Nguyen 4

1Faculty of BiotechnolgyBiotechnology, Vietnam National University of Agriculture, Hanoi 131000, Vietnam
2Faculty of Biotechnology, Vietnam National University of Agriculture, Hanoi 131000, Vietnam
3Institute of Marine Environment and Resources, Hai Phong 181810, Vietnam
4Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi 122000, Vietnam
Received: Oct 17, 2018 /
Revised: Jul 12, 2021 /
Accepted: Oct 22, 2018 /
Published: Oct 22, 2018

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Abstract

Lien Minh chicken is an indigenous breed with several favorable properties, such as high productivity and good meat quality, and is associated with the economic development of the people in the Lien Minh village, Cat Hai, Hai Phong. The objective of the current research was to investigate the single nucleotide polymorphisms (SNPs) of candidate genes, which might be associated with broodiness and egg production traits. Ninety Lien Minh chicken individuals were genotyped for five SNPs of chicken prolactin (PRL), Vasoactive Intestinal Peptide (VIP), neuropeptide Y gene (NPY), growth hormone (GH), and growth hormone receptor (GHR) genes. Blood samples were used for DNA extraction and then for genotyping by the PCR-RFLP method. The allele frequencies obtained were as follows: 0.19 and 0.81 for alleles C and T (PRL-C2161G), respectively; in VIP (G5138982T), 0.55 for the G allele and 0.45 for T; in GH-SacI (the intron 4), 0.02 for A and 0.98 for B; and in GHR-NspI, 0.82 for C and 0.18 for T. The NPY gene (four nucleotide indel) had the frequencies of 0.86 for I and 0.14 for D. The four studied polymorphic loci (PRL, VIP, NPY, and GH) were in Hardy-Weinberg equilibrium in the Lien Minh chicken population. These are the initial results, which can be used to analyze the correlation of molecular markers and egg production traits in Lien Minh chickens.

Keywords: Lien Minh chicken, PCR-RFLP, candidate genes, single nucleotide polymorphisms

Article Details

How to Cite
Tran, B. N., Nguyen, D., Vu, Q., Pham, G., Nguyen, L., Dinh, N. T., & Nguyen, D. T. (2018). Polymorphism Candidate Genes of Lien Minh Chickens. Vietnam Journal of Agricultural Sciences, 1(2), 174-181. https://doi.org/10.31817/vjas.2018.1.2.07

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