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Establishment of Reciprocal Micrografting of Tomato (Solanum lycopersicum L.) and Eggplant (Solanum melongena L.)
and
1Department of Plant Biotechnology, Faculty of Biotechnology, Vietnam National University of Agriculture, Hanoi 131000, Vietnam
2Vietnam Likado Joint Stock Company, Hanoi 127000, Vietnam
Received: Oct 19, 2018 /
Revised: Jul 13, 2021 /
Published: Oct 22, 2018
Main Article Content
Abstract
Micrografting can be used as a key tool to investigate gene-function, long-distance signal transduction, or metabolite movement in different developmental and physiological stages. In plant production, plant grafting can be applied to improve productivity and/or increase the tolerance of plants to stresses. Here, we describe a simple and high efficiency protocol for reciprocal micrografting of tomato (Solanum lycopersicum L.) and eggplant (Solanum melongena L.). Tomato and eggplant seeds can be disinfected with 0.5% Presept for 20 min before germinating on MS media. Seedlings of 5-day-old tomatoes and 15-day-old eggplants were suitable for preparation of scions and rootstocks. Scions were cut into 0.5-1 cm lengths for micrografting. Sucrose levels greatly influenced the graft success rate of all graft combinations including of self- and reciprocal micrografting between tomato and eggplant. While self-grafted tomatoes or eggplants required 20 g L-1 sucrose to get the highest grafting success rate (72% for tomato and 100% for eggplant), reciprocal micrografting of tomato/eggplant and eggplant/tomato reached the highest success rate (83%) on MS medium supplemented with 30 g L-1 sucrose. Grafted plants should be cultured under the illumination conditions of a 16 h light/8 h dark cycle for optimal growth and quality.
Keywords:
micrografting, grafting, tomato (Solanum lycopersicum L.), eggplant (Solanum melongena L.)
Article Details
How to Cite
Dinh, S., & Tran, T. (2018). Establishment of Reciprocal Micrografting of Tomato (Solanum lycopersicum L.) and Eggplant (Solanum melongena L.). Vietnam Journal of Agricultural Sciences, 1(2), 156-165. https://doi.org/10.31817/vjas.2018.1.2.05
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