Effects of Caffeine on In Vitro Fertilization of Pig Follicular Oocytes

Thuong Thi Nguyen 1 , Dat Tien Nguyen 2 , Hanh Van Nguyen 3 , Duc Huu Nguyen 1 and Linh Viet Nguyen 3

1Faculty of Biotechnology, Vietnam National University of Agriculture, Hanoi 131000, Vietnam
2Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi 122100, Vietnam
3Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi 122100, Vietnam - Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Hanoi 122100, Vietnam
Received: Oct 19, 2018 /
Revised: Jul 13, 2021 /
Published: Oct 22, 2018

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Abstract

In the present study, experiments were conducted to assess the effects of caffeine on in vitro fertilization of pig follicular oocytes. Cumulus-oocyte complexes (COCs) were collected from porcine ovaries from slaughterhouses, cultured in in vitro maturation medium 1 (IVM1) at 38.5oC for 20-22 hours and then in in vitro maturation medium 2 (IVM2) for the next 24 hours. Only the oocytes with expanded cumulus cells were selected for in vitro fertilization. Boar frozen semen was used for the porcine IVF procedure. The spermatozoa were pre-incubated in modified TCM 199 medium and subsequently incubated for 3 hours in porcine fertilization medium (pig FM) supplemented with either 2 mM or 5 mM of caffeine. They were cultured in IVC1 medium supplemented with pyruvate and lactate from day 0 to day 2, and then in IVC2 medium supplemented with glucose from day 2 to day 6. The results indicate that pig FM containing 5 mM caffeine gave a higher penetration rate than 2 mM caffeine (33.4% vs. 11.4%, respectively). The blastocyst rates of the two groups were not significantly different (8.4% and 8.0%). In conclusion, a higher caffeine concentration in the fertilization medium may ensure the production of in vitro porcine embryos with acceptable productivity utilizable for further studies on this subject.

Keywords: Caffeine, pig oocytes, in vitro fertilization, IVM, IVC

Article Details

How to Cite
Nguyen, T., Nguyen, D., Nguyen, H., Nguyen, D., & Nguyen, L. (2018). Effects of Caffeine on In Vitro Fertilization of Pig Follicular Oocytes. Vietnam Journal of Agricultural Sciences, 1(2), 182-186. https://doi.org/10.31817/vjas.2018.1.2.08

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