Article Sidebar
Views
924
Downloads
202
Development of a Dot-Elisa Assay for Diagnosis of Southern Rice Black-Streaked Dwarf Disease in the Field
1Faculty of Biotechnology, Vietnam National University of Agriculture, Hanoi 131000, Vietnam
2Faculty of Chemical Technology, Hanoi University of Industry, Hanoi 143510, Vietnam
3Department of Molecular Pathology, Agricultural Genetics Institute, Hanoi 143510, Vietnam
Received: Aug 9, 2021 /
Revised: Sep 30, 2022 /
Published: Sep 30, 2022
Main Article Content
Abstract
Outbreaks of the Southern rice black-streaked dwarf virus (SRBSDV) have caused significant losses in many rice-growing areas in Vietnam, especially in both North and Central Vietnam in recent years. To detect the virus, traditional reverse transcription polymerase chain reaction (RT-PCR) methodology and immunoassays are currently employed. RT-PCR is accurate but requires expensive chemicals and instruments, as well as complex procedures that limit its applicability for field tests. To develop a cheaper, simpler, and reliable SRBSDV diagnosis assay based on the dot-enzyme-linked immunosorbent assay (dot-ELISA) method, anti-SRBSDV polyclonal antibodies were produced by using the antigens derived from the P10 coat protein of SRBSDV, which was achieved from a previous study. The IgG antibody purified from the antiserum of recombinant P10-immunized mice by protein A-agarose affinity chromatography could specifically detect both the target protein and SRBSDV at a dilution of 1:100000. In the trial test of SRBSDV diagnosis, the dot-ELISA assay using the obtained anti-SRBSDV antibody showed an accuracy rate of 90.9% in comparison with the standard RT-PCR assay. These results are important premises for the large-scale application of dot-ELISA assay for SRBSDV diagnosis in order to protect rice crops against viral disease damage.
Keywords:
Antibody, diagnosis, dot-ELISA, P10 protein, SRBSDV
Article Details
How to Cite
Duy, L., Hanh, D., Huong, B., Hang, P., Cuong, H., Hoi, P., Hieu, D., & Phuong, N. (2022). Development of a Dot-Elisa Assay for Diagnosis of Southern Rice Black-Streaked Dwarf Disease in the Field. Vietnam Journal of Agricultural Sciences, 5(3), 1563-1570. https://doi.org/10.31817/vjas.2020.5.3.05
Download Citation
References
-
Ahmed M. S., Khalid A. A., Adel A. R., Hossam S. E. B. & Sherif M. E. G. (2019). Production of polyclonal antibodies to some potato viruses using synthetic peptides. Fresenius Environmental Bulletin. 28: 4870-4877.
Plant Protection Department - Vietnam's Ministry of Agriculture and Rural Development (2017). Announcement of pest situation for the third week of September. Retrieved from https://www.ppd.gov.vn/thong-bao-tinh-hinh-svgh-7-ngay/thong-bao-tinh-hinh-dich-hai-tuan-3-thang-9x002f;2017.html on September 25, 2020 (in Vietnamese).
Gallo Y., Gutiérrez P. A. & Marín M. (2013). Detection of PMTV using polyclonal antibodies raised against a capsid-specific peptide antigen. Medellin Journal National School of Agronomy, 66(2): 6999-7008.
Hanh T. D., Hang T. P., Thanh Ha N., Ha T. T. N., Huong P. T. T, Xuan Hoi P. & Phuong N. D (2020). Production of peptide-specific antibody against protein P10 of Southern rice black-streaked dwarf virus. Journal of Biotechnology. 19(3): 1-11.
Hanh T. D., Minh N. A., Cuu N. V., Huong P. T. T, Xuan Hoi P. & Phuong N. D. (2021a). Generation of synthetic peptide-specific antibody for development of southern rice black-streaked dwarf virus diagnostic test. Vietnam Journal of Agricultural Sciences. 4(3): 1176-1184.
Hanh T. D., Hang P. T., Ha N. T., Ha N. T. T., Huong P. T. T., Xuan Hoi P. & Phuong N. D (2021b). Production of peptide-specific antibody against protein P10 of Southern rice black-striked dwarf virus. Journal of Biotechnology. 19(4): 687-693.
Hanh T. D., Van T. P. & Xuan Hoi P. (2015). Production of polyclonal anti-southern-rice-black-streaked-dwarf-virus antibody using recombinant protein. Vietnam Journal of Agricultural Sciences. 23: 38-43.
Ji Y. H., Gao R. Z., Zhang Y., Cheng Z. B., Zhou T., Fan Y. J. & Zhou Y. J. (2011). A simplified method for quick detection of rice black-streaked dwarf virus and Southern rice black-streaked dwarf virus. Chinese Journal of Rice Science. 25: 91-94.
Li S., Zhang T., Zhu Y., Zhou G. (2017). Co-infection of two reoviruses increases both viruses accumulation in rice by up-regulating of viroplasm components and movement proteins bilaterally and RNA silencing suppressor unilaterally. Virology Journal. 14: 150.
Manoharan S., Parthiban M., Prabhakar T. G., Ravikumar G., Koteeswaran A., Chandran N. D. J. & Rajavelu G. (2004). Rapid serological profiling by an immunocomb-based dot-enzyme-linked immunosorbent test for three major poultry diseases. Veterinary Research Communications. 28: 339 -346.
Michael G. P., Ruta H. & Wayne T. H. (1984). Standardization of the dot enzyme-linked immunosorbent assay (dot-ELISA) for human visceral leishmaniasis. American Journal of Tropical Medicine and Hygiene. 33: 1105-1111.
Miyoshi S., Tokunaga S., Ozawa T., Takeda H., Aono M., Miyoshi T., Kishi H., Muraguchi A., Shimizu S., Nozawa A. & Sawasaki T. (2020). Production of a rabbit monoclonal antibody for highly sensitive detection of citrus mosaic virus and related viruses. PLoS ONE. 15(4): e0229196.
Phuong N. D., Hang T. P., Huong P. T. T, Quyen L. C., Van T. P., Xuan Hoi P. & Hanh T. D. (2020). Production of polyclonal anti-southern-rice-black-streaked-dwarf-virus antibody using recombinant peptide. Vietnam Journal of Agricultural Sciences. 11: 12-19.
Sambrook J. & Russell D. W. (2001). Molecular Cloning: A Laboratory Manual (3rd ed). Cold Spring Harbor Laboratory. Cold Spring Harbor, NY.
Tam T. P., Van T. P., Quang H. N., Phuong N. D. & Xuan Hoi P. (2013). Expression and purification of P10 coat protein of southern rice black-streaked dwarf virus in E. coli. Vietnam Journal of Agricultural Sciences. 24: 35-40.
Wang K., Zheng J. J., Zhang S. G. & Zhou G. H. (2010). Seedling test confirms Southern rice black-streaked dwarf virus cannot be transmitted via rice seed. Guangdong Agricultural Sciences. 7: 95-96.
Wang Z. C., Yu D. D., Li X. Y., Zeng M. J., Chen Z., Bi L. A., Liu J. J, Jin L. H., Hu D. Y., Yang S. & Song B. A. (2012). The development and application of a dot-ELISA assay for diagnosis of Southern rice black-streaked dwarf disease in the field. Viruses. 4: 167-183.
Zhou G. H., Wen J. J. & Cai D. J. (2008). Southern rice black-streaked dwarf virus: A new proposed Fijivirus species in the family Reoviridae. Chinese Science Bulletin. 53: 3677-3685.
Zhou G. H., Zhang S. G., Zou S. F., Xu Z. W. & Zhou Z. Q. (2010a). Occurrence and damage analysis of a new rice dwarf disease caused by Southern rice black-streaked dwarf virus. Plant Protection. 36: 144-146.
Zhou Q., Zhu J. Z., Liang J. G., Chen X. Y. & Gao B. D. (2010b). Rapid detection of southern rice black-streaked dwarf virus. Genomics Applied Biotechnology. 29: 1009-1012.
Zhou G., Xu D., Xu D. & Zhang M. (2013). Southern rice black-streaked dwarf virus: a white-backed planthopper-transmitted fijivirus threatening rice production in Asia. Frontiers in Microbiology. 4: 270.