Article Sidebar
Views
2346
Downloads
518
A Practical and Efficient Method for the Micropropagation of Japanese Cherry (Prunus sp.)
,
Ninh Thi Thao
1
and
1Department of Plant Biotechnology, Faculty of Biotechnology, Vietnam National University of Agriculture, Hanoi 131000, Vietnam
2K55CNSH, Faculty of Biotechnology, Vietnam National University of Agriculture, Hanoi 131000, Vietnam
3VinEco Agricultural Investment Development and Production Limited Liability Company, Hanoi 131000, Vietnam
Received: Feb 15, 2019 /
Revised: Jul 13, 2021 /
Published: Feb 16, 2019
Main Article Content
Abstract
This study was conducted to establish the procedure for in vitro propagation of Japanese cherry (Prunus sp.) to produce large quantity of plantlets and initial planting materials for climate adaptation research of this plant in Hanoi. Single nodal stems were used as the primary explants and initially produced shoots on MS medium supplemented with 1 mg L-1 BA. The highest shoot multiplication rate (9.57 times) was obtained on MS medium containing 1 mg L-1 BA and 0.25 mg L-1 a-NAA after 8 weeks of culture. 100% of the shoots produced roots with a mean of 10.10 roots per plant within 4 weeks on ½ MSM medium with 4 mg L-1 IBA. The survival rate of in vitro derived plantlets after a 6 to 7-week-period of rooting during acclimatization using a soil: coco peat: smoked rice husks (2:2:1, v/v/v) substrate was 100% and acclimatized plantlets showed good growth and development. This is the first report on a practical and efficient in vitro multiplication protocol for Japanese cherry in Vietnam, starting from shoot initiation to establishment of plants under greenhouse conditions.
Keywords:
Japanese cherry, micropropagation, Prunus sp.
Article Details
How to Cite
Thuy Linh, N., Ngoc, P., Thao, N., & Phuong Thao, N. (2019). A Practical and Efficient Method for the Micropropagation of Japanese Cherry (Prunus sp.). Vietnam Journal of Agricultural Sciences, 1(4), 261-269. https://doi.org/10.31817/vjas.2018.1.4.02
Download Citation
References
-
Anh N. T. L., Thanh H. T. T., Phuong N. T. T. and Hung N. T. (2009). Study on in vitro culture of Nhat Tan peach. Journal of Science and Development. Vol 7 (4). pp. 387-393 (in Vietnamese).
Amzallag G. N., Lerner H. R. and Poljakoff-Mayber A. (1992). Interaction between mineral nutrients, cytokinin and gibberellic acid during growth of sorghum at high NaCl salinity. Journal of Experimental Botany. Vol 43 (246). pp. 81-87.
Choudhary R., Chaudhury R., Malik S. K. and Sharma K. C. (2015). An efficient regeneration and rapid micropropagation protocol for almond using dormant axillary buds as explants. Indian Journal of Experimental Biology. Vol 53 (7). pp. 462-467.
Ďurkovič J. (2006). Rapid micropropagation of mature wild cherry. Biologia Plantarum. Vol 50 (4). pp. 733-736.
Duta M. (2008). Research concerning the behavior of the Prunus serrulata “Kwanzan” variety in the process of in vitro reproduction. Bulletin UASVM, Horticulture. Vol 65. pp. 84-89.
Faisal M., Ahmad N., Anis M., Alatar A. A. and Qahtan A. A. (2018). Auxin-cytokinin synergism in vitro for producing genetically stable plants of Ruta graveolens using shoot tip meristems. Saudi Journal of Biological Sciences. Vol 25 (2). pp. 273-277.
Fatima N., Ahmad N. and Anis M. (2011). Enhanced in vitro regeneration and change in photosynthetic pigments, biomass and proline content in Withania somnifera L. (Dunal) induced by copper and zinc ions. Plant Physiology and Biochemistry. Vol 49 (12). pp. 1465-1471.
Fatima N. and Anis M. (2012). Role of growth regulators on in vitro regeneration and histological analysis in Indian ginseng (Withania somnifera L.) Dunal. Physiology and molecular biology of plants: an international journal of functional plant biology. Vol 18 (1). pp. 59-67.
Fotopoulos S. and Sotiropoulos T. E. (2005). In vitro rooting of PR 204/84 rootstock (Prunus persica x P. amygdalus) as influenced by mineral concentration of the culture medium and exposure to darkness for a period. Agronomy Research. Vol 3 (1). pp. 3-8.
Harada H. and Murai Y. (1996). Micropropagation of Prunus mume. Plant Cell, Tissue and Organ Culture. Vol 46. pp.105-119.
Hartman H. T., Kester D. E., Davies F. T. and Geneve R. L. (2010). Hartmann and Kester’s Plant Propagation: Principles and Practices. NJ, USA, Prentice Hall.
Hasan S. Z. U., Ahmad T., Hafiz I. A. and Hussain A.
(2010). Direct plant regeneration from leaves of Prunus rootstock GF-677 (Prunus amygdalus x P. persica). Pakistan Journal of Botany. Vol 42 (6). pp. 3817-3830.
Hrusa F., Ertter B., Sanders A., Leppig G. and Dean E. (2002). Catalogue of non-native vascular plants occurring spontaneously in California beyond those addressed in The Jepson manual Part I. Madrono. Vol 49 (2). pp. 61-98.
Kalinina A. and Brown D. C. W. (2007). Micropropagation of ornamental Prunus spp. and GF305 peach, a Prunus viral indicator. Plant Cell Reports. Vol 26 (7). pp. 927-935.
Mahdavian M., Bouzari N. and Abdollahi H. (2011). Effects of media and plant growth regulators on micropropagation of a dwarfing cherry rootstock (PHL-A). Biharean Biologist. Vol 5 (2). pp. 86-90.
Mansseri-Lamrioui A., Louerguioui A., Bonaly J., Yakoub-Bougdal S., Allili N. and Gana-Kebbouche S. (2011). Proliferation and rooting of wild cherry: The influence of cytokinin and auxin types and their concentration. African Journal of Biotechnology. Vol 10 (43). pp. 8613-8624.
Murashige T. and Skoog F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum. Vol 15 (3). pp. 473-497.
Tančeva C. O. and Kajba D. (2016). Micropropagation of wild cherry (Prunus avium L.) from a clonal seed orchard. Šumarski list. Vol 140 (5-6). pp. 273-281.
Yildirim H., Onay A., Tilkat E. and Akturk Z. (2011). Micropropagation of the apricot (Prunus armeniaca L.) cv. Hacıhaliloğlu by means of single node culture. Turkish Journal of Agriculture and Forestry. Vol 35. pp. 55-64.
Zou Y. N. (2010). Micropropagation of Chinese plum (Prunus salicina Lindl.) using mature stem segments. Notulae Botanicae Horti Agrobotanici Cluj-Napoca. Vol 38 (3). pp. 214-218.