Vol 3 No 1 (2020): Vietnam Journal of Agricultural Sciences

Issue Information:

Starting in 2018, VJAS is set to launch a full English version of VJAS and publish quarterly both online and in print with the ISSN 2588-1299. Each issue will contain 5-10 articles, with each article being approximately 5000-8000 words. All submissions will be independently refereed by editorial board members and will undergo rigorous peer-review following international standards from at least two assigned peer reviewers (including one Vietnamese reviewer and one foreign reviewer) who come from universities and research institutes in Vietnam and other countries around the world. The aim of VJAS is to be indexed in ISI/Scopus lists within four to six years from its first published issue (September 2018).

The English version of VJAS is devoted to publishing papers, both scientific articles and reviews or mini-reviews, from all areas related to agriculture disciplines, especially those focusing on crop science, plant protection, animal science, veterinary medicine, aquaculture, agricultural mechanical and electrical engineering, soil science and plant nutrition, ecology and environment, biotechnology, food technology, economics and rural development, and information technology in agriculture, etc.

DOI: https://doi.org/10.31817/vjas.2020.3.1

Published: 2020-06-05

Impact of Farming Models on the Reproductive Performance and Egg Quality of Vietnamese Local Chicken Breeds: Ho and Dong Tao

Impact of Farming Models on the Reproductive Performance and Egg Quality of Vietnamese Local Chicken Breeds

by Nguyen Van Duy, Hoang Ngoc Mai, Nguyen Dinh Tien, Nguyen Thi Phuong, Vu Dinh Ton
Received: Jan 2, 2020 / Published: Jul 22, 2020
Abstract: The study was conducted from October 2016 to December 2017 at the Experimental Farm, Faculty of Animal Science, Vietnam National University of Agriculture, with the aim to evaluate the interaction between farming model types and reproductive performance of Ho and Dong Tao chickens. The two farming model types used in this research were (1) the traditional type – backyard and (2) the ameliorate type – battery cage. Data were collected from 61 Ho hens and 61 Dong Tao hens continuously during
52 laying weeks. The total number of eggs laid by hens raised in battery cages averaged 88.47 eggs (for Ho hens) and 94.91 eggs (for Dong Tao hens), 35-38% higher than the number of eggs laid by hens raised in a traditional model. The FCR index per 10 eggs laid in battery cages was significantly lower than that in the back-yard model (P< 0.001). In both the Ho and Dong Tao breeds, the rate between the number of embryonated eggs by number of incubated eggs recorded in the ameliorate model was higher than that recorded in the traditional model (P< 0.01 for Ho chickens, P< 0.001 for Dong Tao). The weight of eggs produced by hens raised in battery cages was higher than eggs laid by free-range hens for both Ho and Dong Tao chickens (P< 0.01). As such, the reproductive performances and egg quality of both Ho and Dong Tao breeds were apparently improved when chickens were raised in battery cages.

Successful Application of Site-directed Mutagenesis Polymerase Chain Reaction to Mutate TMS 11 of the Staphylococcal Multidrug Efflux Protein QacA

Successful Application of Site-directed Mutagenesis Polymerase Chain Reaction

by Nguyen Thi Hang, Vu Duc Hanh, Dam Van Phai, Lai Thi Lan Huong, Nguyen Thi Phuong, Le Van Truong, Mohsen Chitsaz, Melissa H Brown
Received: Mar 14, 2019 / Published: Aug 12, 2020
Abstract: Staphylococcus aureus is a major problem in both the clinical setting and within the community. S. aureus can quickly develop resistance to a wide range of antibiotics through a number of different mechanisms, of which, using transporters located in the cell membrane to pump antibiotics out of the cell is the most serious concern. In staphylococcal species, QacA, one such important transporter, is encoded by qacA. QacA is 55kD in size and has 14 transmembrane segments (TMS) (TMS 1-TMS 14). This
research describes the mutation process of the amino acid residues in TMS 11 of QacA using site-direct PCR. In this research, 15 primers were successfully designed for site-directed mutagenesis PCR. The site-mutagenesis PCR was successfully conducted to create 15 qacA mutants. These mutants will be used in further functional research of QacA.

Effects of Frozen Storage on the Physical Properties and Sensory Acceptability of Goat’s Milk Yoghurt

Effects of Frozen Storage on the Physical Properties and Sensory Acceptability of Goat’s Milk Yoghurt

by Nguyen Duc Doan, Vicky Ann Solah
Received: Feb 14, 2019 / Published: Jun 5, 2020
Abstract: The effects of frozen storage of goat’s milk on the physicochemical, physical properties, and sensory attributes of goat’s milk yoghurts were evaluated. Four yoghurts were made from goat’s milk stored at 4℃ overnight, and at -6, -20, or -35℃ for 1 month. Goat’s milk yoghurts were stored at 6℃ for 21 days. Protein and lipid contents in all the yoghurts were insignificantly different, however, the total solids content, including the added sugar in the yoghurts made from frozen
goat’s milk, significantly increased in comparison with that in the yoghurt made from chilling goat’s milk. The water holding capacity (WHC) and viscosity for all the yoghurts at any period of cold storage were statistically different. No significant differences in pH or titratable acidity for all the yoghurts were observed after 1 day of cold storage; however, these physicochemical properties for the yoghurts after 7 days of storage were significantly different. All the yoghurts after 21 days of storage received similar scores for appearance and texture, but significantly different scores for color, odor, flavor, and overall acceptability. 

Expression of Zinc Finger Protein Zat12 from Arabidopsis thaliana in Escherichia coli

Expression of Zinc Finger Protein Zat12 from Arabidopsis thaliana in Escherichia coli

by Le Thi Tuyet Cham, Vu Ngoc Thang, Tran Anh Tuan, Vu Thi Thuy Hang
Received: Oct 26, 2018 / Published: Jul 22, 2020
Abstract: The C2H2 zinc finger protein ZAT12 has been classified as a plant core abiotic stress response gene in the early  response to multiple stresses. ZAT12 links the iron deficiency and oxidative stress responses through the direct interaction with/and negative regulation of a central regulator - FIT. For further research on the regulation of the ZAT12 protein in planta, a huge quantity of ZAT12 proteins is required to inject into mice for the generation of ZAT12 antiserum. In this study, the
gene encoding the ZAT12 protein from Arabidopsis thaliana was cloned into the expression vector - pETBlue-2 and then overexpressed in E. coli T7. A high expression level was indicated by SDS-PAGE. Immunoblot demonstrated successful expression using a bacterial expression system.