Detection of Ascaris suum in the Livers of Chickens Infected Naturally by the Nested Multiplex PCR Assay

Date Received: May 26, 2020

Date Published: Nov 27, 2020

DOI: https://doi.org/10.31817/vjas.2020.3.2.04

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Issue: Vol. 3 No. 2 (2020): Vietnam Journal of Agricultural Sciences

Section:

ANIMAL SCIENCE – VETERINARY MEDICINE – AQUACULTURE

How to Cite:

Yen, N., Hop, N., Thanh, T., Phuong, N., Chien, N., Linh, B., & Dung, D. (2020). Detection of Ascaris suum in the Livers of Chickens Infected Naturally by the Nested Multiplex PCR Assay. Vietnam Journal of Agricultural Sciences, 3(2), 606–611. https://doi.org/10.31817/vjas.2020.3.2.04

Detection of Ascaris suum in the Livers of Chickens Infected Naturally by the Nested Multiplex PCR Assay

Nguyen Thi Hoang Yen (*) 1 , Nguyen Thi Hop 2 , Tran Hai Thanh 1 , Nguyen Van Phuong 1 , Nguyen Thi Hong Chien 1 , Bui Khanh Linh 1   , Do Trung Dung 2

  • Corresponding author: nthyen@vnua.edu.vn
  • 1 Faculty of Veterinary Medicine, Vietnam National University of Agriculture, Hanoi 131000, Vietnam
  • 2 Department of Parasitology, National Institute of Malariology Parasitology and Entomology, Hanoi 100000, Vietnam

    • Keywords

    Ascaris suum, chicken liver, nested multiplex PCR, white spot lesions

    Abstract


    This study was conducted to detect Ascaris suum, Toxocara canis, and Toxocara cati in naturally infected chicken livers, and then to provide information related to ascarid infections in humans. Ninety-four chicken liver samples collected at a fresh market were used for this study. DNA was extracted from each minced liver sample (500 mg) by the alkaline lysis method using NaOH 50mM and Tris-HCl (pH 8.0). The nested multiplex PCR assay using ascarid universal primers was applied to amplify the ITS1 ribosomal RNA gene of A. suum, Toxocara canis, and Toxocara cati in all the liver samples. Then, species specific-primers were used to discriminate between A. suum and Toxocara spp. The results showed that 2 of the 94 chicken liver samples detected positive for the presence of A. suum DNA. This study provided useful information and evidence about A. suum infection in humans via exposure to contaminated soil or eating raw/undercooked chicken livers in Vietnam.

    Author Biography

    Nguyen Van Phuong, Faculty of Veterinary Medicine, Vietnam National University of Agriculture, Hanoi 131000, Vietnam

    Department of Veterinary Parasitology

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