Detection of Ascaris suum in the Livers of Chickens Infected Naturally by the Nested Multiplex PCR Assay

Date Received: May 26, 2020

Date Published: Nov 27, 2020

DOI: https://doi.org/10.31817/vjas.2020.3.2.04

Views

1553

Download

578

Issue: Vol. 3 No. 2 (2020): Vietnam Journal of Agricultural Sciences

Section:

ANIMAL SCIENCE – VETERINARY MEDICINE – AQUACULTURE

How to Cite:

Yen, N., Hop, N., Thanh, T., Phuong, N., Chien, N., Linh, B., & Dung, D. (2020). Detection of Ascaris suum in the Livers of Chickens Infected Naturally by the Nested Multiplex PCR Assay. Vietnam Journal of Agricultural Sciences, 3(2), 606–611. https://doi.org/10.31817/vjas.2020.3.2.04

Detection of Ascaris suum in the Livers of Chickens Infected Naturally by the Nested Multiplex PCR Assay

Nguyen Thi Hoang Yen (*) 1 , Nguyen Thi Hop 2 , Tran Hai Thanh 1 , Nguyen Van Phuong 1 , Nguyen Thi Hong Chien 1 , Bui Khanh Linh 1   , Do Trung Dung 2

  • Corresponding author: nthyen@vnua.edu.vn
  • 1 Faculty of Veterinary Medicine, Vietnam National University of Agriculture, Hanoi 131000, Vietnam
  • 2 Department of Parasitology, National Institute of Malariology Parasitology and Entomology, Hanoi 100000, Vietnam

    • Keywords

    Ascaris suum, chicken liver, nested multiplex PCR, white spot lesions

    Abstract


    This study was conducted to detect Ascaris suum, Toxocara canis, and Toxocara cati in naturally infected chicken livers, and then to provide information related to ascarid infections in humans. Ninety-four chicken liver samples collected at a fresh market were used for this study. DNA was extracted from each minced liver sample (500 mg) by the alkaline lysis method using NaOH 50mM and Tris-HCl (pH 8.0). The nested multiplex PCR assay using ascarid universal primers was applied to amplify the ITS1 ribosomal RNA gene of A. suum, Toxocara canis, and Toxocara cati in all the liver samples. Then, species specific-primers were used to discriminate between A. suum and Toxocara spp. The results showed that 2 of the 94 chicken liver samples detected positive for the presence of A. suum DNA. This study provided useful information and evidence about A. suum infection in humans via exposure to contaminated soil or eating raw/undercooked chicken livers in Vietnam.

    Author Biography

    Nguyen Van Phuong, Faculty of Veterinary Medicine, Vietnam National University of Agriculture, Hanoi 131000, Vietnam

    Department of Veterinary Parasitology

    References

    Azizi S., Oryan A., Sadjjadi S. M. & Zibaei M. (2007). Histopathologic changes and larval recovery of Toxocara cati in experimentally infected chickens. Parasitology Research. 102: 47-52.

    Beaver P. C, Snyder H., Carrera G. M., Dent J. H. & Lafferty J. W. (1952). Chronic eosinophilia due to visceral larvae migrans. Pediatrics. 9: 7-19.

    Campos-da-Silva D. R., da Paz J. S., Fortunato V. R., Beltrame, M. A., Valli L. C. & Pereira F. E. (2015). Natural infection of free-range chickens with the ascarid nematode Toxocara sp, Parasitology Research. 114(11): 4289-4293.

    Choi D., Lim J. H., Choi D. C., Lee K. S., Paik S. W., Kim S. H. & Huh S. (2012). Transmission of Toxocara canis via ingestion of raw cow liver: A cross-sectional study in healthy adults. Korean Journal of Parasitology. 50: 23-27.

    Chomel B. B., Kasten R., Adams C., Lambillotte D., Theis J., Goldsmith R., Koss J., Chioino C., Widjana D. P. & Sutisna P. (1993). Serosurvey of some major zoonotic infections in children and teenagers in Bali, Indonesia. Southeast Asian Journal of Tropical Medicine Public Health. 24: 321-326.

    Do T. D., Tran T. D., Nguyen T. H., Hoang Q. V., Do T. T. T. & Nguyen T. L. A. (2016). Current status of Toxocara spp. infection in humans in some study sites of Hanoi and Hung Yen, 2014-2015. Journal of Malaria and Parasitic Disease Control. 6(102): 10-16 (in Vietnamese).

    Hoenigl M., Valentine T., Zollner-schwetz I., Salzer H. J. F., Raggam R. B., Strenger V. & Krause R. (2010). Pulmonary ascariasis: Two cases in Austria ANS review of the literature. Wiener klinische Wochenschrift. 122: 94-96.

    Inatomi Y., Murakami T., Tokunaga M., Ishiwata K., Nawa Y. & Uchino M. (1999). Encephalopathy caused by visceral larva migrans due to Ascaris suum. Journal of the Neurological Science. 164: 195-196.

    Izumikawa K., Kohno Y., Izumikawa K., Hara H., Hayashi H., Maruyama H. & Kohno S. (2011). Eosinophilic pneumonia due to visceral larva migrans possibly caused by Ascaris suum: A case report and review of recent literatures. Japanese Journal of Infectious Diseases. 64: 428-432.

    Lamberton P. H. L. & Jourdan P. M. (2015). Human Ascariasis: Diagnostics update. Current Tropical Medicine Reports. 2: 189-200.

    Magnaval J. F., Michault A., Calon N. & Charlet J. P. (1994). Epidemiology of human toxocariasis in La Reunion. Transactions of the Royal Society of Tropical Medicine and Hygiene. 88: 531-533.

    Maruyama H., Nawa Y., Noda S., Mimori T. & Choi W. Y. (1996). An outbreak of visceral larva migrans due to Ascaris suum in Kyushu, Japan. The Lancet. 347: 1766-1767.

    Miller L. A., Colby K., Manning S. E., Hoenig D., McEvoy E., Montgomery S. & Sears S. (2015). Ascariasis in humans and pigs on small-scale farms, maine, USA, 2010-2013. Emerging Infectious Diseases. 21: 332-334.

    Le T. H., Nguyen T. L. A., Nguyen T. K., Nguyen T. B. N., Do T. T. T. & Gasser R. B. (2016). Toxocara malaysiensis infection in domestic cats in Vietnam – A emerging zoonotic issue? Infection, Genetic and Evolution. 37: 94-98.

    Nguyen Y. T. H., Wang Z. Z., Maruyama H., Horii Y., Nonaka N. & Yoshida A. (2016). Evaluation of real-time PCR assay for the detection of Ascaris suum contamination in meat and organ meats. Journal of Food Safety. 37(2): 1-6

    Okoshi S. & Ushui M. (1968). Experimental Studies on Toxascaris Leonina. VI. Experimental Infection of Mice, Chicken and Earthworms with Toxascaris leonina, Toxocara canis and Toxocara cati. Japanese

    Journal of Veterinary Medicine Science. 30: 151-166.

    Pinelli E., Herremans T., Harms M. G., Hoek D. & Kortbeek L. M. (2011). Toxocara and Ascaris seropositivity among patients suspected of visceral and ocular larva migrans in the netherlands: Trends from 1998 to 2009. European Journal of Clinical Microbiology & Infectious Diseases: Official Publication of the European Society of Clinical Microbiology. 30: 873-879.

    Umehara F., Ookatsu H., Hayashi D., Uchida A., Douchi Y., Kawabata H. & Osame M. (2006). MRI studies of spinal visceral larva migrans syndrome. Journal of Neurological Science. 249: 7-12.

    Sakakibara A., Basa K., Niwa S., Yagi T., Wakayama H., Yoshida K. & Kimura E. (2002). Visceral larva migrans due to Ascaris suum which presented with eosinophilic pneumonia and multiple intra-hepatic lesions with severe eosinophil infiltration- outbreak in a Japanese area other than Kuyshu. Internal Medicine. 41: 574-579.

    Taira K., Permin A. & Kapel C. M. O. (2003). Establishment and migration pattern of Toxocara canis larvae in chickens. Parasitology Research. 90: 521-523.

    Thompson D. E., Bundy D. A. P., Cooper E. S. & Schantz P. M. (1986). Epidemiological characteristics of Toxocara canis infection of children in a Carribean community. Bull WHO. 64: 283-290.

    Tokojima M., Ashitani J. & Nakazato M. (2004). A case of eosinophilic pneumonia caused by visceral larva migrans due to Ascaris suum. The Japanese Association for Infectious Diseases, 78, 1036–1040.

    Tran T. D. (2014). Actual situation study, some factors has risk to infect larva of Toxocara canis in human and effect of treatment by Albendazole at 2 commune of An Nhon District, Binh Dinh Province (2011-2012). PhD thesis (in Vietnamese).

    Vu T. K. V., Tran M. T. & Dang T. T. S. (2007). A survey of manure management on pig farms in Northern Viennam. Livestock Science. 112: 288-297.

    Wang Z. Z., Shibata M., Nguyen Y. T. H., Hayata Y., Nonaka N., Maruyama H. & Yoshida A. (2018). Development of nested multiplex polymerase chain reaction (PCR) assay for the detection of Toxocara canis, Toxocara cati and Ascaris suum contamination in meat and organ meats. Parasitology International. 67: 622-628.

    Yoshihara S., Hattori J., Nishizono K., Kawamura A., Shimozaki K., Nishida Y. & Hirayama N. (2008). Hepatic lesions caused by migrating larvae of Ascaris suum in chickens. Journal of Veterinary Medicine Science. 70: 1129-1131.