Effects of Caffeine on In Vitro Fertilization of Pig Follicular Oocytes

Date Received: Oct 19, 2018

Date Accepted: Oct 22, 2018

Date Published: Oct 22, 2018

DOI: https://doi.org/10.31817/vjas.2018.1.2.08

Views

2460

Download

483

Issue: Vol. 1 No. 2 (2018): Vietnam Journal of Agricultural Sciences

Section:

ENGINEERING AND TECHNOLOGY

How to Cite:

Nguyen, T., Nguyen, D., Nguyen, H., Nguyen, D., & Nguyen, L. (2018). Effects of Caffeine on In Vitro Fertilization of Pig Follicular Oocytes. Vietnam Journal of Agricultural Sciences, 1(2), 182–186. https://doi.org/10.31817/vjas.2018.1.2.08

Effects of Caffeine on In Vitro Fertilization of Pig Follicular Oocytes

Thuong Thi Nguyen (*) 1 , Dat Tien Nguyen 2 , Hanh Van Nguyen 3 , Duc Huu Nguyen 1   , Linh Viet Nguyen 3

  • Corresponding author: nvlinh@ibt.ac.vn
  • 1 Faculty of Biotechnology, Vietnam National University of Agriculture, Hanoi 131000, Vietnam
  • 2 Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi 122100, Vietnam
  • 3 Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi 122100, Vietnam - Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Hanoi 122100, Vietnam

    • Keywords

    Caffeine, pig oocytes, in vitro fertilization, IVM, IVC

    Abstract


    In the present study, experiments were conducted to assess the effects of caffeine on in vitro fertilization of pig follicular oocytes. Cumulus-oocyte complexes (COCs) were collected from porcine ovaries from slaughterhouses, cultured in in vitro maturation medium 1 (IVM1) at 38.5oC for 20-22 hours and then in in vitro maturation medium 2 (IVM2) for the next 24 hours. Only the oocytes with expanded cumulus cells were selected for in vitro fertilization. Boar frozen semen was used for the porcine IVF procedure. The spermatozoa were pre-incubated in modified TCM 199 medium and subsequently incubated for 3 hours in porcine fertilization medium (pig FM) supplemented with either 2 mM or 5 mM of caffeine. They were cultured in IVC1 medium supplemented with pyruvate and lactate from day 0 to day 2, and then in IVC2 medium supplemented with glucose from day 2 to day 6. The results indicate that pig FM containing 5 mM caffeine gave a higher penetration rate than 2 mM caffeine (33.4% vs. 11.4%, respectively). The blastocyst rates of the two groups were not significantly different (8.4% and 8.0%). In conclusion, a higher caffeine concentration in the fertilization medium may ensure the production of in vitro porcine embryos with acceptable productivity utilizable for further studies on this subject.

    References

    Cheng W. T. R., Moor R. M. and Polge C. (1986). In vitro fertilization of pig and sheep oocytes matured in vivo and in vitro. Theriogenology. Vol 25. pp. 146.

    Kikuchi K., Onishi A., Kashiwazaki N., Iwamoto M., Noguchi J., Kaneko H., Akita T. and Nagai T. (2002). Successful piglet production after transfer of blastocysts produced by a modified in vitro system. Biology of Reproduction. Vol 66. pp. 1033-1041.

    Nagai T., Miura K., Kikuchi K. and Okamura N. (1993). Effects of Caffeine on In-Vitro Fertilization of Pig Follicular Oocytes. Journal of Reproduction and Development. Vol 39. pp. 347-352.

    Nagai T., Takahashi T., Masuda H., Shioya Y., Kuwayama M., Fukushima M., Iwasaki S. and Hanada A. (1988). In vitro fertilization of pig oocytes by frozen boar spermatozoa. Journal of Reproduction and Fertility. Vol 84. pp. 585-591.

    Thoa N. T., Anh L. N., Huong V. T., Ha T. S., Huong D. V. and Huong N. T. (2009). Porcine embryo production by in vitro fertilization of oocytes matured in vitro using NCSU-37 supplemented with 10% porcine follicular fluid (PFF). National institute of animal sciences Annual Report. Vol 19. pp. 34-40 (in Vietnamese).

    Suzuki M., Misumi K., Ozawa M., Noguchi J., Kaneko H., Ohnuma K., Fuchimoto D., Onishi A., Iwamoto M. and Saito N. (2006). Successful piglet production by IVF of oocytes matured in vitro using NCSU-37 supplemented with fetal bovine serum. Theriogenology. Vol 65. pp. 374-386.