Development of a Dot-Elisa Assay for Diagnosis of Southern Rice Black-Streaked Dwarf Disease in the Field

Date Received: Aug 09, 2021

Date Published: Sep 30, 2022

DOI: https://doi.org/10.31817/vjas.2020.5.3.05

Views

1207

Download

268

Issue: Vol. 5 No. 3 (2022): Vietnam Journal of Agricultural Sciences

Section:

ENGINEERING AND TECHNOLOGY

How to Cite:

Duy, L., Hanh, D., Huong, B., Hang, P., Cuong, H., Hoi, P., … Phuong, N. (2022). Development of a Dot-Elisa Assay for Diagnosis of Southern Rice Black-Streaked Dwarf Disease in the Field. Vietnam Journal of Agricultural Sciences, 5(3), 1563–1570. https://doi.org/10.31817/vjas.2020.5.3.05

Development of a Dot-Elisa Assay for Diagnosis of Southern Rice Black-Streaked Dwarf Disease in the Field

La Duc Duy (*) 1 , Do Thi Hanh 2 , Bui Thi Thu Huong 1 , Pham Thu Hang 3 , Ha Viet Cuong 1 , Pham Xuan Hoi 3 , Dam Quang Hieu 3   , Nguyen Duy Phuong 3

  • Corresponding author: phuongnd.bio@gmail.com
  • 1 Faculty of Biotechnology, Vietnam National University of Agriculture, Hanoi 131000, Vietnam
  • 2 Faculty of Chemical Technology, Hanoi University of Industry, Hanoi 143510, Vietnam
  • 3 Department of Molecular Pathology, Agricultural Genetics Institute, Hanoi 143510, Vietnam

    • Keywords

    Antibody, diagnosis, dot-ELISA, P10 protein, SRBSDV

    Abstract


    Outbreaks of the Southern rice black-streaked dwarf virus (SRBSDV) have caused significant losses in many rice-growing areas in Vietnam, especially in both North and Central Vietnam in recent years. To detect the virus, traditional reverse transcription polymerase chain reaction (RT-PCR) methodology and immunoassays are currently employed. RT-PCR is accurate but requires expensive chemicals and instruments, as well as complex procedures that limit its applicability for field tests. To develop a cheaper, simpler, and reliable SRBSDV diagnosis assay based on the dot-enzyme-linked immunosorbent assay (dot-ELISA) method, anti-SRBSDV polyclonal antibodies were produced by using the antigens derived from the P10 coat protein of SRBSDV, which was achieved from a previous study. The IgG antibody purified from the antiserum of recombinant P10-immunized mice by protein A-agarose affinity chromatography could specifically detect both the target protein and SRBSDV at a dilution of 1:100000. In the trial test of SRBSDV diagnosis, the dot-ELISA assay using the obtained anti-SRBSDV antibody showed an accuracy rate of 90.9% in comparison with the standard RT-PCR assay. These results are important premises for the large-scale application of dot-ELISA assay for SRBSDV diagnosis in order to protect rice crops against viral disease damage.

    References

    Ahmed M. S., Khalid A. A., Adel A. R., Hossam S. E. B. & Sherif M. E. G. (2019). Production of polyclonal antibodies to some potato viruses using synthetic peptides. Fresenius Environmental Bulletin. 28: 4870-4877.

    Plant Protection Department - Vietnam's Ministry of Agriculture and Rural Development (2017). Announcement of pest situation for the third week of September. Retrieved from https://www.ppd.gov.vn/thong-bao-tinh-hinh-svgh-7-ngay/thong-bao-tinh-hinh-dich-hai-tuan-3-thang-9x002f;2017.html on September 25, 2020 (in Vietnamese).

    Gallo Y., Gutiérrez P. A. & Marín M. (2013). Detection of PMTV using polyclonal antibodies raised against a capsid-specific peptide antigen. Medellin Journal National School of Agronomy, 66(2): 6999-7008.

    Hanh T. D., Hang T. P., Thanh Ha N., Ha T. T. N., Huong P. T. T, Xuan Hoi P. & Phuong N. D (2020). Production of peptide-specific antibody against protein P10 of Southern rice black-streaked dwarf virus. Journal of Biotechnology. 19(3): 1-11.

    Hanh T. D., Minh N. A., Cuu N. V., Huong P. T. T, Xuan Hoi P. & Phuong N. D. (2021a). Generation of synthetic peptide-specific antibody for development of southern rice black-streaked dwarf virus diagnostic test. Vietnam Journal of Agricultural Sciences. 4(3): 1176-1184.

    Hanh T. D., Hang P. T., Ha N. T., Ha N. T. T., Huong P. T. T., Xuan Hoi P. & Phuong N. D (2021b). Production of peptide-specific antibody against protein P10 of Southern rice black-striked dwarf virus. Journal of Biotechnology. 19(4): 687-693.

    Hanh T. D., Van T. P. & Xuan Hoi P. (2015). Production of polyclonal anti-southern-rice-black-streaked-dwarf-virus antibody using recombinant protein. Vietnam Journal of Agricultural Sciences. 23: 38-43.

    Ji Y. H., Gao R. Z., Zhang Y., Cheng Z. B., Zhou T., Fan Y. J. & Zhou Y. J. (2011). A simplified method for quick detection of rice black-streaked dwarf virus and Southern rice black-streaked dwarf virus. Chinese Journal of Rice Science. 25: 91-94.

    Li S., Zhang T., Zhu Y., Zhou G. (2017). Co-infection of two reoviruses increases both viruses accumulation in rice by up-regulating of viroplasm components and movement proteins bilaterally and RNA silencing suppressor unilaterally. Virology Journal. 14: 150.

    Manoharan S., Parthiban M., Prabhakar T. G., Ravikumar G., Koteeswaran A., Chandran N. D. J. & Rajavelu G. (2004). Rapid serological profiling by an immunocomb-based dot-enzyme-linked immunosorbent test for three major poultry diseases. Veterinary Research Communications. 28: 339 -346.

    Michael G. P., Ruta H. & Wayne T. H. (1984). Standardization of the dot enzyme-linked immunosorbent assay (dot-ELISA) for human visceral leishmaniasis. American Journal of Tropical Medicine and Hygiene. 33: 1105-1111.

    Miyoshi S., Tokunaga S., Ozawa T., Takeda H., Aono M., Miyoshi T., Kishi H., Muraguchi A., Shimizu S., Nozawa A. & Sawasaki T. (2020). Production of a rabbit monoclonal antibody for highly sensitive detection of citrus mosaic virus and related viruses. PLoS ONE. 15(4): e0229196.

    Phuong N. D., Hang T. P., Huong P. T. T, Quyen L. C., Van T. P., Xuan Hoi P. & Hanh T. D. (2020). Production of polyclonal anti-southern-rice-black-streaked-dwarf-virus antibody using recombinant peptide. Vietnam Journal of Agricultural Sciences. 11: 12-19.

    Sambrook J. & Russell D. W. (2001). Molecular Cloning: A Laboratory Manual (3rd ed). Cold Spring Harbor Laboratory. Cold Spring Harbor, NY.

    Tam T. P., Van T. P., Quang H. N., Phuong N. D. & Xuan Hoi P. (2013). Expression and purification of P10 coat protein of southern rice black-streaked dwarf virus in E. coli. Vietnam Journal of Agricultural Sciences. 24: 35-40.

    Wang K., Zheng J. J., Zhang S. G. & Zhou G. H. (2010). Seedling test confirms Southern rice black-streaked dwarf virus cannot be transmitted via rice seed. Guangdong Agricultural Sciences. 7: 95-96.

    Wang Z. C., Yu D. D., Li X. Y., Zeng M. J., Chen Z., Bi L. A., Liu J. J, Jin L. H., Hu D. Y., Yang S. & Song B. A. (2012). The development and application of a dot-ELISA assay for diagnosis of Southern rice black-streaked dwarf disease in the field. Viruses. 4: 167-183.

    Zhou G. H., Wen J. J. & Cai D. J. (2008). Southern rice black-streaked dwarf virus: A new proposed Fijivirus species in the family Reoviridae. Chinese Science Bulletin. 53: 3677-3685.

    Zhou G. H., Zhang S. G., Zou S. F., Xu Z. W. & Zhou Z. Q. (2010a). Occurrence and damage analysis of a new rice dwarf disease caused by Southern rice black-streaked dwarf virus. Plant Protection. 36: 144-146.

    Zhou Q., Zhu J. Z., Liang J. G., Chen X. Y. & Gao B. D. (2010b). Rapid detection of southern rice black-streaked dwarf virus. Genomics Applied Biotechnology. 29: 1009-1012.

    Zhou G., Xu D., Xu D. & Zhang M. (2013). Southern rice black-streaked dwarf virus: a white-backed planthopper-transmitted fijivirus threatening rice production in Asia. Frontiers in Microbiology. 4: 270.