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• Detection of Ascaris suum in the Livers of Chickens Infected Naturally by the Nested Multiplex PCR Assay

  Nguyen Thi Hoang Yen, Nguyen Thi Hop, Tran Hai Thanh, Nguyen Van Phuong, Nguyen Thi Hong Chien, Bui Khanh Linh, Do Trung Dung

  Received: May 26, 2020 / Published: Nov 27, 2020

   Abstract PDF

  This study was conducted to detect Ascaris suum, Toxocara canis, and Toxocara cati in naturally infected chicken livers, and then to provide information related to ascarid infections in humans. Ninety-four chicken liver samples collected at a fresh market were used for this study. DNA was extracted from each minced liver sample (500 mg) by the alkaline lysis method using NaOH 50mM and Tris-HCl (pH 8.0). The nested multiplex PCR assay using ascarid universal primers was applied to amplify the ITS1 ribosomal RNA gene of A. suum, Toxocara canis, and Toxocara cati in all the liver samples. Then, species specific-primers were used to discriminate between A. suum and Toxocara spp. The results showed that 2 of the 94 chicken liver samples detected positive for the presence of A. suum DNA. This study provided useful information and evidence about A. suum infection in humans via exposure to contaminated soil or eating raw/undercooked chicken livers in Vietnam.

• Identification of Three Toxocara species, T. canis in Dogs, and T. cati and T. malaysiensis in cats, in Vietnam by PCR-RFLP Analysis and DNA Sequencing

  Nguyen Thi Hoang Yen, Nguyen Van Phuong, Duong Duc Hieu, Nguyen Thi Lan Anh

  Received: Jul 25, 2022 / Published: Dec 29, 2023

   Abstract PDF

  Toxocara canis and T. cati are common roundworms parasitizing dogs and cats, respectively. However, a recent study detected T. malaysiensis, but not T. cati, in cats from some parts of Ha Noi and Nam Dinh Provinces raising the question of whether T. cati is present in cats in Vietnam. This study was conducted to determine the composition of Toxocara species in dogs and cats in Vietnam. One hundred and twenty-seven Toxocara adult worms were collected from dogs and cats and were analyzed by PCR-RFLP assay and DNA sequencing of a partial section of the cox1 gene. As a result, all samples amplified by PCR showed bands about 430 bp in length. PCR products digested by MseI from isolates from cats and dogs showed four and two restriction patterns, respectively. The six different patterns were chosen to sequence. Based on the restriction patterns and sequencing results, two Toxocara species were identified in the cats, namely T. cati (110/111 isolates) and T. malaysiensis (1/111 isolates) collected from Ha Noi and Hai Duong, respectively; and T. canis was identified in the dogs (16/16 isolates). In addition, the present study indicated intra-specific sequence variations of Toxocara spp. in dogs and cats. In conclusion, the study confirmed the presence of both T. cati and T. malaysiensis in cats, and T. canis in dogs in Vietnam, and suggests further large-scale investigations to fully understand the distribution and genetic variations of Toxocara spp. in cats and dogs in Vietnam.