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• Genetic Diversity of Chanos Chanos (Forsskål, 1775) from Natural Populations in Vietnam

  Vu Thi Trang, Tran Thi Thuy Ha, Tran Thi Kim Ngan, Nguyen Dinh Vinh, Pham Hong Nhat, Vu Thi Huyen

  Received: Jul 14, 2021 / Accepted: Jun 27, 2022 / Published: Jun 27, 2022

   Abstract PDF

  The genetic diversity of five natural populations of milkfish (Chanos chanos) collected in Nghe An, Quang Binh, Binh Dinh, Phu Yen, Khanh Hoa provinces in Vietnam was examined using COI gene sequence analysis. Twelve haplotypes were noted from a total of 50 sequences along with 12 variable sites and 6 parsimony informative sites. The Quang Binh milkfish population had the highest haplotype (0.889 ± 0.060) and nucleotide diversities (0.00301 ± 0.00049). Overall, haplotype and nucleotide diversities were 0.804 ± 0.036 and 0.00212 ± 0.00026, respectively. Genetic differentiation (FST) was high between the milkfish populations of Nghe An – Quang Binh (0.21744) and Nghe An - Phu Yen (0.26215). Haplotype network analysis indicated that milkfish populations shared common haplotypes and each population had its own private haplotypes. Population structure and demographic expansion were not evident for all populations except for Quang Binh. This is the first principal endeavor to understand genetic information of milkfish in Vietnam, thereby providing information for scientists, managers, and the general public to establish timely strategies to explore, protect, and develop milkfish genetic resources in the future.

• Genetic Divergence of Cultivated Rice Varieties in North Vietnam for Grain Quality Traits Using D2 Cluster Analysis

  Nguyen Ba Thang

  Received: Oct 21, 2021 / Published: Jun 27, 2022

   Abstract PDF

  The study was carried out with twenty-four genotypes of rice during the Spring season, 2020 in Hanoi, Vietnam in order to exploit the attributes and divergence of quality properties from existing rice cultivars in North Vietnam. The rice genotypes were characterized based on ten quality parameters of milling, physical appearance and physiochemical characters using Mahalanobis D2 statistic. D2 analysis allocated the 24 genotypes into eight clusters, of which cluster I was the largest with 12 genotypes. The clustering showed the random distribution of genotypes into different clusters indicating that the origin and release duration were not related to the genetic diversity of grain quality. Among different traits, amylose content, length/width ratio and gel consistency were identified as topmost contributors in total divergence. Cluster I possessed a maximum intra-cluster value of 296.34 and the maximum inter-cluster distance was recorded between cluster IV and VI (2247.39) succeeded by cluster I and VI (2245.94) designing the members presented in these clusters can be exploited as potential donors in a breeding program to result good physicochemical quality traits in rice.

• Nitrogen-Use Efficiency Evaluation and Genome Survey of Vietnamese Rice Landraces (Oryza sativa L.)

  Hanh Thi Thuy Nguyen, Thuy Linh Mai Dinh, Trung Quoc Nguyen, Cuong Van Pham

  Received: Oct 19, 2018 / Accepted: Oct 22, 2018 / Published: Oct 22, 2018

   Abstract PDF

  The overuse of fertilizers can result in many adverse effects such as decreasing fertilizer use efficiency of plants, wasting resources, increasing farming costs, and polluting our environment. Local rice landraces including indigenous and local rice varieties, may contain considerable genetic diversity that can serve as sources of germplasm for genetic improvements of nutrient use efficiency, yield, resistance to pests and pathogens, and important agronomic traits. Increasing the fertilizer use efficiency of crops by developing new rice varieties is necessary for sustainable agriculture. In this study, six rice varieties, Chiem Tay (CT), Te Tep (TT), Re Bac Ninh (RB), IR24, P6DB, and Khang Dan 18 (KD18), were evaluated for nitrogen use efficiency. Two landraces, P6DB and CT, which showed the lowest and highest values of nitrogen use efficiencies, were selected for a genome survey. Ninety-seven out of the 1051 surveyed markers indicated polymorphisms. These polymorphic markers were distributed along to each of the 12 chromosomes and were either scattered quite evenly on a chromosome or were condensed at particular regions in the physical map. The obtained information on nitrogen use efficiency (NUE) variation and the marker map should be very useful to further identify QTLs/genes involving in NUE as well as other genetic analyses toward the development of sustainable agriculture.