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• Growth Characterization of Sugarcane (Saccharum spp.) under Salinity and Drought Stresses at the Seedling Stage

  Le Thi Tuyet Cham, Vu Khanh Linh, Vu Thi Thuy Hang

  Received: Dec 19, 2023 / Accepted: Aug 26, 2024 / Published: Sep 30, 2024

   Abstract PDF

  This study aimed to assess the combined effects of salinity and drought stresses on the growth and physiology of sugarcane. The pot experiment was carried out in the Autumn cropping season of 2021 under the polyhouse conditions at the Vietnam National University of Agriculture. The experiment consisted of four treatments: non-stress treatment (control), drought stress, salt stress, and salt and drought stress (combined stress). Five weeks after transplanting, salt stress was applied first for four weeks and followed by drought stress for another two weeks. The results showed that under the impact of stresses, sugarcane growth was inhibited with decreases in plant height, number of leaves, Fv/Fm, SPAD, and the fresh and dry weights of roots and stems. The growth and physiology indicators were the lowest under the combined effects of salinity and drought stress.

• Comparative Analysis of an Integral Component of Bacterial Cell Division from the Lactococcusand BacillusGenera

  Tong Van Hai, Vu Ngoc Lan, Pham Thu Ha, Le Thi Van, Tran Thi Hong Hanh, Bui Thi Thu Huong, Nguyen Hai Phuong, Ha Thi Quyen, Ha Duc Chu

  Received: Dec 30, 2022 / Published: Dec 29, 2023

   Abstract PDF

  FtsZ proteins have been well-characterized to play a crucial role in cell division. Unfortunately, data are scarce on FtsZ proteins in the Lactococcus and Bacillus genera. The objective of this study was to analyze the features of FtsZ proteins in the Bacillus and Lactococcus genus groups. By exploring the available genomes, we identified and characterized FtsZ proteins in 19 Bacillus and 22 Lactococcus species. The sizes and weights of the FtsZ proteins ranged from 376 to 410 aa residues and 39.53 to 44.15 kDa in the Bacillus genus, respectively, and from 387 to 430 aa residues and  41.14 to 45.11 kDa in the Lactococcus genus, respectively. All the FtsZ proteins in the Bacillus and Lactococcus species were acidic and globular, and localized in the cytoplasm. Next, 3D modeling and multiple alignments were performed. We realized that the FtsZ proteins in the Bacillus and Lactococcus species exhibited five specific regions. Taken together, our study could provide a general background for further functional characterization of the FtsZ proteins in Bacillus and Lactococcus species.

• A Review on Potential Application of CRISPR/Cas Systems in the Improvement of the Growth Habits and Fruit Quality of Tomato (Solanum lycopersicum) in Vietnam

  Van Hai Tong, Trinh Thi Thu Thuy, Phan Thi Hien, Chu Duc Ha, La Viet Hong, Tran Van Tien, Nguyen Quoc Trung

  Received: Aug 16, 2021 / Published: Mar 30, 2022

   Abstract PDF

  Tomato (Solanum lycopersicum) is known as the most important vegetable crop that is widely cultivated throughout the world. Improvements of the growth, development, and productivity have become core strategies for the sustainable development of tomato in many countries. Here, we performed an intensive summary of recent applications of genome editing in customizing the growth habits and fruit quality in tomato plants. First, the advantages of genome editing, particularly CRISPR/Cas systems, were introduced. We then summarized all up-to-date studies related to the genome editing-based functional characterization of genes of interest in tomato with the aim of designing the growth habits and enhancing the fruit quality. Finally, we discussed the potential applications of this promising tool in tomato breeding programs in Vietnam. Taken together, our review has provided a wide view for further studies towards improving the growth, development, and productivity of tomato in Vietnam.

• Chemical constituents of leaves Dialium cochinchinense Pierre

  Vu Thi Huyen, Doan Thi Thuy Ai, Nguyen Thi Hien

  Received: Sep 30, 2020 / Published: Oct 28, 2021

   Abstract PDF

  The genus Dialium belongs to the Caesalpinioideae family, consisting of approximately 30 species distributed in the tropical regions. Secondary metabolites from the  Dialium genus have been reported to exhibit various biological activities including antioxidant, cytotoxicity and antimicrobial activities. This work describes the isolation and characterization of five compounds from the leaves of Dialium cochinchinense Pierre. Their structures were established by spectroscopic analysis, including MS and NMR spectra. Accordingly, the isolated compounds were identified to be lupeone (1), b-sitostenone (2), β-sitosterol (3), daucosterol (4), and dihydrokaempferide (5). To the best of our knowledge, this is the first report of the isolation of compounds 1 and 5 from the genus Dialium.

• Characterization of Variovorax Strain C6d Isolated from the Algae-bacteria Consortia

  Vu Thi Hoan

  Received: Sep 24, 2020 / Published: Oct 29, 2021

   Abstract PDF

  The rhizosphere microorganisms can form beneficial, pathogenic, or neutral relationships. These relationships can promote plant growth and productivity. Among them, a number of Variovorax isolates from the rhizosphere were isolated. Bacteria Variovorax strain C6d (AB552893) was isolated from the non axenic culture of Chlorella spp., C6. The cell was Gram-negative, motile, non-spore-forming, short and rod-shaped (0.5-1.0x1.5-2.0µm). Colonies were in white colour after 7 days on 10-fold diluted Nutrient Broth. The strain was able to tolerate NaCL to 1.0% but not to 4.0% of NaCl. It grew quite well at temperatures ranging from 10°C to 37°C, yet did not show any growth at 4°C and 42°C. The dominant isoprenoid quinone was ubiquinone 8 (Q8). The major fatty acid composition of this strain was summed feature 3, 16:0 and 18:1:w7. The DNA G+C content of strain C6d was 70.4 mol%.

• Characterization and Identification of a Streptomyces Strain with Biocontrol Activity against Aeromonas Hydrophila Causing Haemorrhage Disease in Fish

  Nguyen Xuan Canh, Dinh Thi Thom, Nguyen Thanh Huyen

  Received: Aug 21, 2018 / Accepted: Aug 21, 2018 / Published: Aug 21, 2018

   Abstract PDF

  In this study, experiments were performed to identify and screen Streptomyces strains that are antagonistic to Aeromonas hydrophila, which causes hemorrhagic disease in fishes. Among 80 isolates, 3 strains capable of antagonizing Aeromonas hydrophila by the agar diffusion plate method were obtained. The strain numbered 1083 had a strong activity with a 25 mm diameter clear zone of bacteria. This strain showed grey colonies after 7 days of incubation. After 7 days of incubation, the grey colonies had white borders, produced soluble pigments on the medium, grew well at 30°C and neutral pH, and adapted well to medium containing a high salt concentration. Strain 1083 was able to utilize different sources of carbon and nitrogen. Sequence analysis of 16S rRNA showed that strain 1083 had a similarity of 99% compared to Streptomyces antibioticus. Based on morphological, physiological, and biochemical characteristics, as well as molecular biological analysis, strain 1083 was identified as Streptomyces antibioticus.